New 3D lighting system (DIY)

Images made through a microscope. All subject types.

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Tonikon
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New 3D lighting system (DIY)

Post by Tonikon »

Hi everyone,
this morning, before cooking for Christmas lunch, I tried to experiment with a 3D light system very different from those commonly used: the main innovation is a crystal dome placed between the light source and the condenser. I share with you my first result ... I hope to be able to get even better results.
Toni

P.S. Microscope is an old black enamel Leitz SM equipped with Berek condenser e Leitz Apo objectives. Lighting system is a home-made led source...
Attachments
ND Leitz Apo 25 3D.jpg

David Sykes
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Re: New 3D lighting system (DIY)

Post by David Sykes »

Very nice image, do you have a comparison photo taken with your 'normal' transmitted-light setup ?

(Hope you enjoyed your Lidl vegan Xmas pudding :-) )

Tonikon
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Re: New 3D lighting system (DIY)

Post by Tonikon »

Hi David,
unfortunately I haven't a comparison image in normal brightfield, but I well remember that the ciliate was almost invisible, diaphanous and transparent like a ghost!
In the next few days, I hope to take off the chef's apron and put on the lab coat. Only then will I be able to make comparison images :-)

dolmadis
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Re: New 3D lighting system (DIY)

Post by dolmadis »

Tonikon wrote:
Fri Dec 25, 2020 5:01 am
Hi everyone,
I tried to experiment with a 3D light system very different from those commonly used: the main innovation is a crystal dome placed between the light source and the condenser. .
Would be interested in seeing the "crystal dome".

BR

John

Tonikon
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Re: New 3D lighting system (DIY)

Post by Tonikon »

dolmadis wrote:
Sat Dec 26, 2020 4:37 am
Would be interested in seeing the "crystal dome".
John
You are right, John...I have used a bombastic name to indicate a simple hemisphere glass, easily available (ebay, Amazon...)
Attachments
PhaCo-Like Setup.jpg
hemisphaere glass.jpg

Saul
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Re: New 3D lighting system (DIY)

Post by Saul »

I used colimating lens, not hemisphere (got this DIC'ish effect) .
Found it useful mixing with other illumination types.

Rotation/displacing helps a lot (if you have enough room between condenser holder and illuminator) .

Short YouTube video
Saul
μ-stuff

Tonikon
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Joined: Thu Oct 30, 2008 2:58 pm
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Re: New 3D lighting system (DIY)

Post by Tonikon »

Saul wrote:
Fri Jan 01, 2021 1:57 pm
I used colimating lens, not hemisphere (got this DIC'ish effect) .
Found it useful mixing with other illumination types.

Rotation/displacing helps a lot (if you have enough room between condenser holder and illuminator) .

Short YouTube video

Ni Saul,
your setup is a lot more "conscious" than mine :)
I randomly played with a glass hemisphere, while you built an excellent system with predictable results.
I will try to get spikes from your system ... unfortunately my Berek condenser is very different from your because the first lens in the optical path is a field lens!
Anyway, in the specific section I just shared a photo taken a couple of days ago with my system ...

Toni

Robert Berdan
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Re: New 3D lighting system (DIY)

Post by Robert Berdan »

Very interesting image - it appears the Dome is forming a type of Oblique lighting. I have never seen this type of Dome used before.

The dome creates a DIC like effect.

I found some for $5.99 on Ali Express for those that might want to try a crystal dome.
https://www.aliexpress.com/i/569515401.html - $5.99 each 20 mm in diameter.


Cheers
RB
Attachments
HTB1sHPWMpXXXXczXFXXq6xXFXXX5.jpg

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