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Stentor coeruleus by various Microscopy techniques

 
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Robert Berdan



Joined: 18 Oct 2017
Posts: 211
Location: Calgary

PostPosted: Tue Mar 31, 2020 9:37 pm    Post subject: Stentor coeruleus by various Microscopy techniques Reply with quote

Fresh water Ciliate: Stentor coeruleus
Used Zeiss Axioscope, Nikon Optiphot and Olympus E microscopes, photographed with Nikon D700, D500 and D800 cameras

Bright field showing basal cup, this ciliate just injested a rotifer visible in vacuole near the foot.



Bright field microscopy 50X


Phase contrast microscopy


DIC microscopy


DIC microscopy showing beaded macronucleus 600X


Stentor stained wiht Acridine orange Fluorescence microscopy 50X
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carlos.uruguay



Joined: 23 Feb 2012
Posts: 5337
Location: Uruguay - Montevideo - America del Sur

PostPosted: Wed Apr 01, 2020 12:40 pm    Post subject: Reply with quote

Marvelous!!!
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sebba28



Joined: 29 Dec 2011
Posts: 290
Location: Poland,Ireland

PostPosted: Wed Apr 01, 2020 3:26 pm    Post subject: Reply with quote

Very nice.
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Sumguy01



Joined: 28 Jan 2013
Posts: 1496
Location: Ketchikan Alaska USA

PostPosted: Wed Apr 01, 2020 8:04 pm    Post subject: Reply with quote

Very nice.
Thanks for sharing.
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Boron



Joined: 18 Oct 2019
Posts: 9

PostPosted: Thu Apr 02, 2020 1:10 pm    Post subject: Reply with quote

Beautiful shots. The fourth one 'DIC microscopy' is dry NA0.9 or oil NA1.4 and stack from how many pictures?
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Robert Berdan



Joined: 18 Oct 2017
Posts: 211
Location: Calgary

PostPosted: Fri Apr 03, 2020 3:56 pm    Post subject: 63X objective Dry - no stacking Reply with quote

The 4th shot is not stacked of the Stentor, neither is the 5th shot showing the macronucleus, it was taken with a 63X objective NA 085 dry Zeiss infinity objective.
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