www.photomacrography.net :: View topic - Acridine Orange staining and Fluorescence Microscopy
www.photomacrography.net Forum Index
An online community dedicated to the practices of photomacrography, close-up and macro photography, and photomicrography.
Photomacrography Front Page Amateurmicrography Front Page
Old Forums/Galleries
 
 FAQFAQ   SearchSearch   MemberlistMemberlist   UsergroupsUsergroups   RegisterRegister 
 ProfileProfile   Log in to check your private messagesLog in to check your private messages   Log inLog in 
Acridine Orange staining and Fluorescence Microscopy

 
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope
View previous topic :: View next topic  
Author Message
Robert Berdan



Joined: 18 Oct 2017
Posts: 211
Location: Calgary

PostPosted: Sun Mar 29, 2020 1:15 pm    Post subject: Acridine Orange staining and Fluorescence Microscopy Reply with quote

I am attaching some images taken with a Fluorescence microscope (Nikon Optiphot) and specimens stained with 1 microgram\ml Acridine orange in water with UV excitation and green (FITC) emission filter. Acridien stains nuclei (DNA) green, RNA and lysosomes (red).

Copepod head


Copepod


Copepod eggs (glow like those from an Alien)


Ribbon worm - showing cells Nuclei Green


Tail end (posterior) of Ribbon worm


Stentor (ciliate) flattened showing Green macronucleus - like beads on a string.
Back to top
View user's profile Send private message Send e-mail Visit poster's website
sebba28



Joined: 29 Dec 2011
Posts: 290
Location: Poland,Ireland

PostPosted: Mon Mar 30, 2020 9:15 am    Post subject: Reply with quote

Very nice.
Back to top
View user's profile Send private message
Display posts from previous:   
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Photography Through the Microscope All times are GMT - 7 Hours
Page 1 of 1

 
Jump to:  
You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot vote in polls in this forum


Powered by phpBB © 2001, 2005 phpBB Group