Depth of field for DIC imaging

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David Sykes
Posts: 42
Joined: Mon May 02, 2016 2:04 pm
Location: North Wales,U.K.

Depth of field for DIC imaging

Post by David Sykes »

I will be making some image stacks using transmitted and epi DIC.
DIC is very useful for its 'optical sectioning' capabilities but I am curious as to whether members use a calculation or just a process of trial-and-error when determining step size.


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