Paramecium

TekaPrzyrodnika · Post by **TekaPrzyrodnika** » Thu Mar 03, 2016 5:43 pm

Hello

I'd like to show you several films with my favourit protist

http://www.youtube.com/watch?v=8MykW-k31Go

Jacek · Post by **Jacek** » Thu Mar 03, 2016 11:11 pm

Very nice

Pau · Post by **Pau** » Fri Mar 04, 2016 6:12 am

Teka, excellent video.

Welcome aboard!

billporter1456 · Post by **billporter1456** » Fri Mar 04, 2016 6:27 am

Welcome to the forum. I love that combination of DF and Pol!

fpelectronica · Post by **fpelectronica** » Fri Mar 04, 2016 11:24 am

Very nice!

TekaPrzyrodnika · Post by **TekaPrzyrodnika** » Fri Mar 04, 2016 11:35 am

Thank you for your kindness and warm welcome

Another film , now for studying them movement

http://www.youtube.com/watch?v=8pDPicc9ats

carlos.uruguay · Post by **carlos.uruguay** » Fri Mar 04, 2016 12:11 pm

Welcome
Nice videos
What microscope and camera do you use?

Protos · Post by **Protos** » Fri Mar 04, 2016 1:17 pm

Welcome here ! very interesting views
I am also curious about video mode (slow motion, etc) and microscopy mode (DIC, Pol, etc..)

TekaPrzyrodnika · Post by **TekaPrzyrodnika** » Sat Mar 05, 2016 2:11 am

Thanks for your positives

Equipment is routine... Old labophot with CFN 40x and camera fujifilm x-t10. I don't have DIC unfirtunatly

Only polarisation and I try use other technique like DF or - like here- oblique light.
Film is also very comon, without slow motion or another effects - just real-time

zzffnn · Post by **zzffnn** » Sat Mar 05, 2016 8:31 am

Welcome to the forum, Teka.

Did you close down condenser iris and/or objective iris for your second video? Or was the contrast effect achieved by filter placement alone?

carlos.uruguay and I routinely use oblique+polarization in our videos too. I don't close down any iris and only achieve contrast with filter placement. I don't know if Carlos close down his iris.

I think for 40x NA 0.65 or lower, oblique+pol won't perform much worse than DIC. DIC pulls ahead at 60x or NA 0.85 and above.

And your second video seems to show Frontonia, though it is a close relative of Paramecium. I could be wrong though.

TekaPrzyrodnika · Post by **TekaPrzyrodnika** » Sat Mar 05, 2016 8:55 am

Welcom again

On the second video effect of oblique light was obtained by using piece of dark paper placed close to iris and iris wasn't close down itself.

Frontonia you say...Mayby you're right. Of course I don't claim that this is Paramecium caudatum (it has characteristic shape) but I thought about Paramecium aurelia or similar. There's several or even ten-odd species od Paramecium but I don't have any knowledge to classify anything :/

billporter1456 · Post by **billporter1456** » Sat Mar 05, 2016 10:32 am

That second video is simply beautiful! Nicely done!!! I don't think it is Paramecium as I didn't see an oral grove or that star-shaped contractile vacuole. And the overall shape didn't look like Paramecium to my old eyes. But it didn't really look like Frontonia either. Maybe Bruce Taylor will chime in and tell us what the creature is. Regardless of classification, I am very impressed with your work!

TekaPrzyrodnika · Post by **TekaPrzyrodnika** » Sat Mar 05, 2016 12:02 pm

Thank you very much

So mayby someone has good knowledge about species of Ciliophora?
I tell you why I thought that this is some species o Paramecium.
This is very very old culture in the jar. I observe only one species of ciliata and one chlorophyta. Ciliata catched in deeper water has slim shape, star-shaped contractile vacuole and low number of (fat?-) globules. On the surface ciliata are fatter but still shaped like the lowers, but wit bigger number of globules (that's why I think that that fat globules bring them to surface). And of course star-shaped contractile vacuole.
Under microscope at the beggining they are normal but when water has evaporised they are getting fatter and they hardly can do any rotation.
Why I don't think that's Frontonia? Because in the weeks when there is apperance milions of chlorophyta they still eats common bacteria. Of course 2-5 algae also some of them but Frontonia seems to not miss a trick to eat any algae they find.

e.g like this (sorry for quality, its from video)

Bruce Taylor · Post by **Bruce Taylor** » Sat Mar 05, 2016 12:30 pm

Lovely videos! The colours are very agreeable.

These appear to be a Paramecium species, somewhat squashed by the coverslip. There are brief glimpses of the cytostome (mouth) as the cell rotates, and it does not resemble that of Frontonia or Ophryoglena. Also, there are two contractile vacuoles.

Pressure increases as water evaporates from the edges of the coverslip, and it is not unusual for a Paramecium to become smooth and oval, with an everted oral groove, like these guys.

TekaPrzyrodnika · Post by **TekaPrzyrodnika** » Mon Mar 07, 2016 12:38 am

Thank you very very much for explanation

It was very helpful

Another movie with sweet Paramecium

http://www.youtube.com/watch?v=tSAxnzl3QXM