http://www.photomacrography.net/forum/v ... php?t=2818
This post illustrates the setup I used for those shots. This is a setup I frequently use to shoot image stacks of still subjects.
The tabletop setup used consists of two primary components… a “stand” for the camera and a “stand” for the subject. The upper image below shows these components. The subject stand (right half) is quite versatile as the subject platform can be raised or lowered and rotated to just about any position via either the stand pole or the small ball-head. The entire piece can be slid around the tabletop.
The camera “stand” will take some explaining (there is something of a method to my madness)
... so from the bottom upward:Three 5lb barbell weights were glued together to provide a solid, heavy, base. (Small plastic tabs attached on the bottom allow it to be slid over the tabletop without scratching the surface). Above that is a leveling attachment… this allows some left/right and forward/backward tilt capability. Above that, attached via Arca-Swiss style clamps and plates, is a focus block from a large Olympus “stand” microscope. The fine focus of this mechanism is what is used to move the camera when shooting image stacks. Then comes a Nikon bellow unit with the D200 attached. On front is a 35mf2.8 Canon micro lens, which has a diffuser (made from a whiffle-ball) attached to the front. In the camera hot shoe is a Nikon SU-800 Wireless to control some of the flash units used.
While this tall combination looks like a recipe for vibration nightmares, the techniques I use for actually taking the images eliminate any problems. There are two approaches I use. If incandescent (continuous) light is used for exposure, it is adjusted so that my exposure times are 2 to 4 seconds in duration. I always use mirror lockup, and any vibration caused by the shutter mechanism quickly damps out at the beginning of the exposure. So effectively, the vast majority of the exposure takes place with everything perfectly still. (All “hardware” connections are metal-to-metal with no cork or rubber anyplace. I’m sort of surprised at how stable it actually is).
If I am using electronic flash for the exposure (as was the case with these aphid shots) I work as follows:
Camera shutter is manually set to one second. Prior to taking the pictures, the ambient light (continuous) is lowered so that no image (or at least a hardly perceptible) image is registered at the 1 second exposure time. The camera flash sync is set to “second shutter curtain”. Mirror lock-up is used, and the flash units are turned on and used at manual settings. When used this way you can be assured of really vibration free results. The mirror is first pre-released. After a pause, the one second exposure begins. By the time the flash fires at the end of the one second exposure everything is dead calm. (This may actually be vibration-reduction “overkill”, but it is easy to do and extremely effective). Since I want the flash units to be fully recharged before the next shot, the time delay between shots works out nicely. (When you use flash to shoot a “stack” you should use a manual flash mode and allow enough time for the flash to recycle to be sure you are getting the same exposure with each frame).
The lower image shows this as they were set for the aphid shots. What is not seen in this shot is that there was a large, slave-fired electronic flash directly overhead pointed down onto the top of the diffusion hemisphere. When the picture is taken, the SU-800 fires two small Nikon SB-R200 flash units on either side of the diffuser. (This also triggers the large slaved flash above). Power levels on all flash units can be adjusted to get different effects. If a unit is positioned very close to the diffuser (as is an SB-R200 seen here) the area of the spherical diffuser that is illuminated can be small, and it is possible to get a soft shadow effect (depending on the power setting of the other flashes).
I hope this doe not seem hopelessly complicated. The reality is this could all probably be done equally well with a much simpler arrangement. But I’ve been “collecting” many of the bits and pieces seen here for over thirty years so I just grab and assemble them as I need. This particular arrangement has proven extremely versatile, but with that versatility comes a bit more complexity.
Spare parts cabinets are 
