* Canon EOS RP. Full frame, 26 MP (6,240 x 4,160), 5.76 µm/px, built-in focus stacking.
* Sigma 150mm 1:2.8 APO EX DG HSM Macro.
* Nikon CFI Plan Achromat 10x (MRL00102). NA 0.25. WD 10.5mm.
Focus stacking works well, both, with the Sigma 150mm alone, or Sigma 150mm + Nikon 10x. I typically shoot indoors, USB tethered with Canon EOS software.
The EOS RP stacking feature uses an electronic shutter. There is practically no vibration except for small lens focus adjustments and I can get up to ~4.8 fps in jpeg-fine mode depending on exposure time per frame (more typically 2 to 3 fps).
Setting the Sigma to f/2.8 to maximize field of view, the Nikon 10x projects a (subjectively) usable image disk of around 30.9mm diameter; or black-to-black ~37.7mm.
The Sigma 150mm lens focus distance indicator goes from 0.38m to infinity. With the Nikon 10x attached, I get approximately 2.3mm subject depth. To avoid large scale differences in stacks, I generally shoot from 0.52m to infinity, which gives me around 1.3mm subject depth. The choice of 0.52m isn't scientific and stacking software might work with a wider or even full range.
The approximate subject size projected onto the width of the sensor (35.94mm) is:
* Sigma set to 0.38m: ~3.6mm (mag ~10x)
* Sigma set to 0.52m: ~4.6mm (mag ~7.86x)
* Sigma set to infinity: ~4.8mm (mag ~7.44x)
Stacking step size can be set ranging from nominal values 1 (small steps) to 10 (large steps), with 4 recommended for normal lens configurations. With the Nikon 10x attached, I generally set this to 7, which gives me around 380 frames from "0.52m" to "infinity", ie, approximately 3.5µm per step. If I set it to 2 (nevere used in practice), I can take 1,000 frames from "0.52m" to "0.6m". If I set it to 1, the Sigma appears to stop moving, or maybe I ran out of patience.
Battery power is a minor limiting factor. I can take several thousand shots, but for longer sessions I need to keep an eye on the battery or need to use a power adaptor. But, to be sure, the ~350 shots per battery charge in the EOS RP spec sheet does not apply when stacking.