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Crab Louse in darkfield and DIC

 
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WalterD



Joined: 06 Jul 2015
Posts: 593
Location: Rotterdam, the Netherlands

PostPosted: Wed Apr 08, 2020 6:18 am    Post subject: Crab Louse in darkfield and DIC Reply with quote

Subject louse (Phthirus pubis) was examined as follows :
1 Darkfield - Leitz NPL Fluotar 6,3x , unmounted
2 DIC - Leitz 10x/0.45 oil immersion, mounted in euparal
3 Leitz NPL Fluotar 6,3x/0.20 in euparal without coverslip
4 Leitz NPL Fluotar 16x/0.45, mounted in euparal
5 Leitz NPL DIC 25x/0.50, mounted in euparal
6 Leitz NPL DIC 100x/1.30, mounted in euparal

2,3 and 4 were taken with non-dedicated DIC objectives, "pseudo DIC".








Thanks for looking.
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houstontx



Joined: 31 Jul 2015
Posts: 295

PostPosted: Wed Apr 08, 2020 1:42 pm    Post subject: Reply with quote

First image is very dramatic! All images are great, I love it. Interested to see your Leitz DIC (ICT?)setup.
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Robert Berdan



Joined: 18 Oct 2017
Posts: 211
Location: Calgary

PostPosted: Fri Apr 10, 2020 3:34 pm    Post subject: Nice pictures Reply with quote

Hi Walter very nice pictures thanks for sharing.
Rob
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WalterD



Joined: 06 Jul 2015
Posts: 593
Location: Rotterdam, the Netherlands

PostPosted: Tue Apr 21, 2020 10:39 pm    Post subject: Reply with quote

Thanks both for your positive remarks.

@Houstonx: below a couple of pictures of my DIC -indeed ICT- setup.
Center is an Ortholux II ( the "new" Ortholux) with an eyepiece head designed for 30mm diameter eyepieces. This results in a field number of 24, where the standard FN is 18. So it catches a larger part of the image.
The revolver and condenser are made for DIC, the condenser also has phase contrast stops that I did not use yet. The objectives were mounted in fixed (optimal) position in the revolver in Leitz factory.
Since a couple of months I've been using a mirror house, which makes it possible to switch between flashlight (speedlite) and 10 W Led.

Regards,

Walter



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Pau
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Joined: 20 Jan 2010
Posts: 5025
Location: Valencia, Spain

PostPosted: Wed Apr 22, 2020 2:08 am    Post subject: Reply with quote

Walter, very nice pictures!

An interesting microscope you have. I see an afocal arrangement. Are you using a Canon pancake 40mm lens over a Periplan 10X eyepiece?
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Pau
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Saul



Joined: 31 Jan 2011
Posts: 1598
Location: Naperville, IL USA

PostPosted: Wed Apr 22, 2020 10:13 am    Post subject: Reply with quote

Impressive photos, very nice light on #1
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Saul
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WalterD



Joined: 06 Jul 2015
Posts: 593
Location: Rotterdam, the Netherlands

PostPosted: Thu Apr 23, 2020 12:13 pm    Post subject: Reply with quote

Hi Pau and Saul, thanks for your positive remarks!
@Pau; yes correct it is an afocal setup, identical to the setup with the Orthoplan I posted a couple of years ago in equipment discussions. The camera lens is indeed a pancake and the camera is hanging on the ceiling with a beamer support, so different microscopes can be positioned underneath it.
The eyepiece is a 6,3x Periplan, the same you've been using in your first post in this forum "Diatom in Darkfield" (2010). That was very helpful to me to arrange my setup.

Regards,

Walter
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Pau
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Joined: 20 Jan 2010
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Location: Valencia, Spain

PostPosted: Fri Apr 24, 2020 7:06 am    Post subject: Reply with quote

Walter, thanks for the follow up, this is an interesting setup, I now remember your original anti-vibration system
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Charles Krebs



Joined: 01 Aug 2006
Posts: 5843
Location: Issaquah, WA USA

PostPosted: Sat Apr 25, 2020 10:27 pm    Post subject: Reply with quote

Nice set! I'm partial to the darkfield myself, very 3-dimensional and great light light on the upper surface (is the body somewhat translucent?)
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WalterD



Joined: 06 Jul 2015
Posts: 593
Location: Rotterdam, the Netherlands

PostPosted: Tue Apr 28, 2020 3:04 am    Post subject: Reply with quote

Hi Charles, thanks for your positive response!

Charles Krebs wrote:
Nice set! I'm partial to the darkfield myself, very 3-dimensional and great light light on the upper surface (is the body somewhat translucent?)


The body is a rather translucent indeed, small insects are therefore good to combine with darkfield. I do like experimenting with darkfield, next post will be diatoms again.

Regards,

Walter
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