www.photomacrography.net :: View topic - 40X NA 0.80 at WD 0.62 mm, shooting the BMSB cuticle
www.photomacrography.net Forum Index
An online community dedicated to the practices of photomacrography, close-up and macro photography, and photomicrography.
Photomacrography Front Page Amateurmicrography Front Page
Old Forums/Galleries
 
 FAQFAQ   SearchSearch   MemberlistMemberlist   UsergroupsUsergroups   RegisterRegister 
 ProfileProfile   Log in to check your private messagesLog in to check your private messages   Log inLog in 
40X NA 0.80 at WD 0.62 mm, shooting the BMSB cuticle

 
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Macro and Micro Technique and Technical Discussions
View previous topic :: View next topic  
Author Message
rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 20622
Location: Richland, Washington State, USA

PostPosted: Thu Jan 23, 2020 5:26 pm    Post subject: 40X NA 0.80 at WD 0.62 mm, shooting the BMSB cuticle Reply with quote

At https://www.photomacrography.net/forum/viewtopic.php?t=40860, I wrote
Quote:
Nikon 40X NA 0.80 M Plan apochromat
...
It required some surgery, which I will describe separately

Here is the surgery that I promised to describe:



I was not able to unscrew the BD illumination collar, so I simply cut it off with a diamond blade in a rotary tool. Macrero showed a similar approach , but I opted to cut off the minimum needed to get a clear light path, while leaving the objective protected by spring loading.

Here is the objective in its pre-surgical state.



The objective gives nominal 0.7 mm working distance. I measure 0.62 mm, using a couple of pieces of aluminum foil and an ohmmeter to detect physical contact.

Here is what the small WD looks like in use, just with the diffuser pulled back.



Setup with this small WD is greatly simplified because many years ago I had bought a 40X NA 0.5 ELWD objective that has the same mount and is parfocal with the NA 0.80. The ELWD gives 9.8 mm WD, so it's simple enough to focus and frame using that, set a hard stop, then back off, switch objectives, advance to the same stop and tweak framing. Without the matching ELWD to set focus and framing at 9.8 mm, working with the 0.62 mm WD would be a lot more challenging.


Just for completeness, here's the overall setup that I used to shoot the cuticle. The hardwired 580EX II provides second curtain sync to use with a long shutter-open time when extra low vibration is needed, with the YN460-II's optically slaved. For the stack shown above, the extra stability was not needed, so I used a 1/200 second shutter time which allowed to leave on the continuous illumination that I had used for setup. According to the setup photo, power setting was 1/16 on the 580EX II, and 1/32 on each of the YN460's.



I hope this is helpful.

--Rik
Back to top
View user's profile Send private message Visit poster's website
David Sykes



Joined: 02 May 2016
Posts: 40
Location: North Wales,U.K.

PostPosted: Mon Jan 27, 2020 4:50 am    Post subject: Reply with quote

How did you setup the objective and rotary tool to get such a clean cut ?

[rummaging through a rarely opened draw last night I found a wooden box containing Zeiss Epiplan HD 40x 0.85 (three off !) and 8x 0.2 together with Epiplan pol 4x 0.1,16x 0.35 and 40x 0.85. Must try these]
Back to top
View user's profile Send private message Visit poster's website
Adalbert



Joined: 30 Nov 2015
Posts: 1024

PostPosted: Mon Jan 27, 2020 7:49 am    Post subject: Reply with quote

Hello Rik,
Why not :-)
In my case it was a NeoSplan 80x / 0.75:
https://www.photomacrography.net/forum/viewtopic.php?t=36045&highlight=
BR, ADi
Back to top
View user's profile Send private message
rjlittlefield
Site Admin


Joined: 01 Aug 2006
Posts: 20622
Location: Richland, Washington State, USA

PostPosted: Wed Jan 29, 2020 1:39 pm    Post subject: Reply with quote

David Sykes wrote:
How did you setup the objective and rotary tool to get such a clean cut ?

I made a small jig from pieces of wood. It comprised a trough for the objective to lie in while being turned on its axis, stops to keep the objective from moving along its axis, and another stop to rest the end of the spindle of the rotary tool against. This gave me enough control to reliably manipulate the objective with one hand while manipulating the rotary tool with the other.

I have a lathe that is suitable for this sort of work, but I decided the chance of disaster was lower with the wood jig and intimate control by hand.

Adalbert wrote:
Hello Rik,
Why not :-)

I'm sorry, I don't understand the question. Why not what?

--Rik
Back to top
View user's profile Send private message Visit poster's website
Adalbert



Joined: 30 Nov 2015
Posts: 1024

PostPosted: Wed Jan 29, 2020 3:43 pm    Post subject: Reply with quote

To saw, but it is not really a question but something like if you have to saw, then you saw it.
Back to top
View user's profile Send private message
Display posts from previous:   
Post new topic   Reply to topic    www.photomacrography.net Forum Index -> Macro and Micro Technique and Technical Discussions All times are GMT - 7 Hours
Page 1 of 1

 
Jump to:  
You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot vote in polls in this forum


Powered by phpBB © 2001, 2005 phpBB Group