Nikon Microphot. DIC Sénarmont
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Nikon Microphot. DIC Sénarmont
I recently purchased a Nikon Microphot microscope equipped with Sénarmont DIC. This system is different from the PZO that it used.
I have done some tests. I appreciate comments, advice and criticisms that help me use it correctly. Thank you
Francisco
P.S.
Both the photographs and the videos are unedited or retouched
Video:
https://youtu.be/6LWn7b7syd8
Video:
https://youtu.be/p52jXIsj6CA
I have done some tests. I appreciate comments, advice and criticisms that help me use it correctly. Thank you
Francisco
P.S.
Both the photographs and the videos are unedited or retouched
Video:
https://youtu.be/6LWn7b7syd8
Video:
https://youtu.be/p52jXIsj6CA
- rjlittlefield
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Re: Nikon Microphot. DIC Sénarmont
I am confused. The still images posted inline are only 227 × 173 pixels, including the black bevel border. Are the images really that small and bordered coming out of the camera?fpelectronica wrote:Both the photographs and the videos are unedited or retouched
--Rik
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Hi Francisco,
Looks OK. There will be differences depending on the type of objectives you use. Do you use immersion for the condenser (assuming it is NA 1.35 or 1.4)?
Have you tried playing around with the contrast levels (rotating the polariser) or do you always work at maximum contrast level?
Regards, Ichty
Looks OK. There will be differences depending on the type of objectives you use. Do you use immersion for the condenser (assuming it is NA 1.35 or 1.4)?
Have you tried playing around with the contrast levels (rotating the polariser) or do you always work at maximum contrast level?
Regards, Ichty
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Re: Nikon Microphot. DIC Sénarmont
The photographs have been resized to be published in the forum because it only supports 150K (I think)rjlittlefield wrote:I am confused. The still images posted inline are only 227 × 173 pixels, including the black bevel border. Are the images really that small and bordered coming out of the camera?fpelectronica wrote:Both the photographs and the videos are unedited or retouched
--Rik
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Thanks for your answer.Ichthyophthirius wrote:Hi Francisco,
Looks OK. There will be differences depending on the type of objectives you use. Do you use immersion for the condenser (assuming it is NA 1.35 or 1.4)?
Have you tried playing around with the contrast levels (rotating the polariser) or do you always work at maximum contrast level?
Regards, Ichty
The condenser is from NA 1.4. I have used the objective Nikon Plan Apo20X.
I used the rotation of the polarizer to obtain different contrast. With crossed polarizers the image is very dark (almost dark field).
I'm trying to make a compensator to try to achieve DIC colors.
With this system the turning of the polarized only gives me "gray scale", not colors. In the PZO system, the displacement of the prism gives me colors.
Regards
Francisco
- Cactusdave
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As far as image size is concerned you can post much larger photos on the forum than your two samples.
As far is DIC is concerned your DIC looks good, especially in the first video. I can't remember the exact implementation of DIC on the Microphot, but on the Diaphot DIC implementation, there is a lever on the condenser which switches in or out a red/magenta compensator plate. With this inserted, rotation of the Nomarski prism adjustment produces all the colour spectrum of 'colour DIC'. Inserting a retarder after the DIC polariser and before the specimen and objective should have the same effect. Layers of Sellotape and various plastic and cellophane sheets all make good retarders for this application as they do in ordinary polarised illumination. I have used this method to get 'colour DIC' with the Zeiss DIC implementation which lacks a built in lambda/compensator plate
http://www.photomacrography.net/forum/v ... hp?p=10#10First, make sure that your image dimensions are 1024 pixels or smaller, on both axes, AND that the image file length is 300 KB or smaller. Use your favorite photo editor to resize the image and set the JPEG compression quality as needed to meet those two rules.
As far is DIC is concerned your DIC looks good, especially in the first video. I can't remember the exact implementation of DIC on the Microphot, but on the Diaphot DIC implementation, there is a lever on the condenser which switches in or out a red/magenta compensator plate. With this inserted, rotation of the Nomarski prism adjustment produces all the colour spectrum of 'colour DIC'. Inserting a retarder after the DIC polariser and before the specimen and objective should have the same effect. Layers of Sellotape and various plastic and cellophane sheets all make good retarders for this application as they do in ordinary polarised illumination. I have used this method to get 'colour DIC' with the Zeiss DIC implementation which lacks a built in lambda/compensator plate
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear
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Thanks for the comment and advice.Cactusdave wrote:As far as image size is concerned you can post much larger photos on the forum than your two samples.
http://www.photomacrography.net/forum/v ... hp?p=10#10First, make sure that your image dimensions are 1024 pixels or smaller, on both axes, AND that the image file length is 300 KB or smaller. Use your favorite photo editor to resize the image and set the JPEG compression quality as needed to meet those two rules.
As far is DIC is concerned your DIC looks good, especially in the first video. I can't remember the exact implementation of DIC on the Microphot, but on the Diaphot DIC implementation, there is a lever on the condenser which switches in or out a red/magenta compensator plate. With this inserted, rotation of the Nomarski prism adjustment produces all the colour spectrum of 'colour DIC'. Inserting a retarder after the DIC polariser and before the specimen and objective should have the same effect. Layers of Sellotape and various plastic and cellophane sheets all make good retarders for this application as they do in ordinary polarised illumination. I have used this method to get 'colour DIC' with the Zeiss DIC implementation which lacks a built in lambda/compensator plate
regards
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