Utricularia - inside the bladder of doom

Images made through a microscope. All subject types.

Moderators: ChrisR, Chris S., Pau, rjlittlefield

blepharopsis
Posts: 148
Joined: Fri Nov 28, 2014 12:39 pm
Location: Virginia, USA
Contact:

Utricularia - inside the bladder of doom

Post by blepharopsis »

Greetings! I'd like to share with you some confocal images of the trap (or bladder) of an aquatic carnivorous plant, Humped bladderwort (Utricularia gibba). Enjoy!

Magnification: 100x
Microscope: Zeiss confocal laser scanning microscope LSM 710.
Objective: 10x, NA 0.45
Laser lines: 405, 488, 562 nm.
Colors: blue and green - cellulose stained with Calcofluor white; red - autofluorescence of chloroplasts.

Image

Image

Image

Image

sebba28
Posts: 296
Joined: Thu Dec 29, 2011 11:13 pm
Location: Poland,Ireland

Post by sebba28 »

Beautiful images.

Sumguy01
Posts: 1531
Joined: Mon Jan 28, 2013 11:05 pm
Location: Ketchikan Alaska USA

Post by Sumguy01 »

:D :smt038 Very interesting pics.
Thanks for sharing.

GemBro
Posts: 261
Joined: Wed Aug 20, 2014 5:20 pm
Location: Surrey [UK]

Post by GemBro »

Blimey :shock: ... fantastic shots ...
Canon 550D(T2i) ML (Nightly Builds) | Canon 5D MKII | Raynox 250 | Palinar 35mm f2.8 (reversed) | EL-Nikkor 50mm f2.8 N | EL-Nikkor 50mm f4 N | EL-Nikkor 50mm f4 | Bellows | Objectives: LOMO 3.7x 0.11 : 8x 0.20 : 40x 0.65
RiG II - 'Bamboo': Olympus CH Focus Block with Inverted Arca/Swiss | Canon 430 EX (x2) | Olympus T20 flash (x2) | Youngnuo YN-622C Wireless triggers (x3) | Ikea Jansjo 3W LED Lighting (x3)
Stepper Motor Focusing System (Helicon Remote)

Cactusdave
Posts: 1631
Joined: Tue Jun 09, 2009 12:40 pm
Location: Bromley, Kent, UK

Post by Cactusdave »

Laser scanning confocal is just great, the red Ferrari supermodel of light microscopy :lol: Great images, no more to say. Didn't I see that one of the second set won an award recently? :wink:
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

ChrisR
Site Admin
Posts: 8557
Joined: Sat Mar 14, 2009 3:58 am
Location: Near London, UK

Post by ChrisR »

Astonishing. Thanks for showing these. A load of questions arise :)

escocat
Posts: 273
Joined: Wed Nov 19, 2014 1:08 pm
Location: Messina - Sicily - Italy

Post by escocat »

The second is a real show :shock:

carlos.uruguay
Posts: 5354
Joined: Thu Feb 23, 2012 8:05 pm
Location: Uruguay - Montevideo - America del Sur
Contact:

Post by carlos.uruguay »

Impressive!!!!
How are the organisms prepared to observe them?

Jacek
Posts: 5353
Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

fabulous pictures, collect jaw from the floor :shock:

arturoag75
Posts: 1597
Joined: Fri Feb 05, 2010 1:05 am
Location: italy
Contact:

Post by arturoag75 »

Absolutely fantastic :shock: :shock:
Arturo

RogelioMoreno
Posts: 2962
Joined: Fri Nov 20, 2009 11:24 am
Location: Panama

Post by RogelioMoreno »

I love all! :shock:

Rogelio

blepharopsis
Posts: 148
Joined: Fri Nov 28, 2014 12:39 pm
Location: Virginia, USA
Contact:

Post by blepharopsis »

carlos.uruguay wrote:Impressive!!!!
How are the organisms prepared to observe them?
Thanks, Carlos. The traps were fixed in 2% formaldehyde/2% glutaraldehyde in pond water, stained with Calcofluor White, embedded in 6% agarose, sectioned with Vibratome @ 0.5 mm in 2 planes (frontal, sagital), mounted in slightly alkaline water.

blepharopsis
Posts: 148
Joined: Fri Nov 28, 2014 12:39 pm
Location: Virginia, USA
Contact:

Post by blepharopsis »

Jacek wrote:fabulous pictures, collect jaw from the floor :shock:
Dziekuje, Jacek! Gdzie w Polsce przebywasz? Ja jestem z Krakowa :)

Olympusman
Posts: 4584
Joined: Sun Jan 15, 2012 12:31 pm

Bladderwort

Post by Olympusman »

Zowie!
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

blepharopsis
Posts: 148
Joined: Fri Nov 28, 2014 12:39 pm
Location: Virginia, USA
Contact:

Post by blepharopsis »

Cactusdave wrote:Laser scanning confocal is just great, the red Ferrari supermodel of light microscopy :lol: Great images, no more to say. Didn't I see that one of the second set won an award recently? :wink:
Yes, LSCM is a powerful tool - but when it comes to output images I'm finding myself drawn to the aesthetics of the Nomarski DIC microscopy lately :)

It was a similar one - see below.

Image

Post Reply Previous topicNext topic