Zerene: How to stop the rotation of the stable subject
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Zerene: How to stop the rotation of the stable subject
Hello everybody,
Does anyone know how to stop the rotation of the subject on the stack?
Originally I tried stacking in one pass, but there were too many artifacts.
So then I tried the sub stacks.
First I aligned the images and then created the sub-stacks.
- Number of single shots =501
- Size of the sub-stack=15, Overlap=5 ; Number of sub-stacks=50, PMax
This is what the single shots look like: And the sub-stacks look like that: BTW, the subject is a slide with a cover glass; magnification 100x.
The photo subject is not rotated on the single shots,
but the dots on the cover glass move.
Best, ADi
Does anyone know how to stop the rotation of the subject on the stack?
Originally I tried stacking in one pass, but there were too many artifacts.
So then I tried the sub stacks.
First I aligned the images and then created the sub-stacks.
- Number of single shots =501
- Size of the sub-stack=15, Overlap=5 ; Number of sub-stacks=50, PMax
This is what the single shots look like: And the sub-stacks look like that: BTW, the subject is a slide with a cover glass; magnification 100x.
The photo subject is not rotated on the single shots,
but the dots on the cover glass move.
Best, ADi
- rjlittlefield
- Site Admin
- Posts: 23625
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
Re: Zerene: How to stop the rotation of the stable subject
Go to Options > Preferences > Alignment and remove the checkmark on Rotation.
For this level of magnification you should also remove the checkmark on Scale.
If your illumination is stable, remove the checkmark on Brightness.
If your rig is stable enough, then remove the checkmarks on Shift X and Shift Y also.
In general, it is better to remove checkmarks on all aspects that are physically stabilized by the apparatus. This prevents the alignment algorithm from being misled by how the size and shape of things appear to change as they go in and out of focus.
--Rik
For this level of magnification you should also remove the checkmark on Scale.
If your illumination is stable, remove the checkmark on Brightness.
If your rig is stable enough, then remove the checkmarks on Shift X and Shift Y also.
In general, it is better to remove checkmarks on all aspects that are physically stabilized by the apparatus. This prevents the alignment algorithm from being misled by how the size and shape of things appear to change as they go in and out of focus.
--Rik
Re: Zerene: How to stop the rotation of the stable subject
Hello Rik,
Thanks for the hint!
The next question is: to rotate or not to rotate.
With the alignment/rotation enabled, the result looks like this:
And with the alignment/rotation disabled, the result looks like that:
I have tried to create a usable stack, but it has been very difficult: Best,
ADi
Thanks for the hint!
The next question is: to rotate or not to rotate.
With the alignment/rotation enabled, the result looks like this:
And with the alignment/rotation disabled, the result looks like that:
I have tried to create a usable stack, but it has been very difficult: Best,
ADi
- rjlittlefield
- Site Admin
- Posts: 23625
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
Re: Zerene: How to stop the rotation of the stable subject
You mentioned that "the dots on the cover glass move". I can see in the top two images of your last post that for sure all that extra moving content would wreak havoc with keeping the subject aligned.
But I am puzzled by what is physically going on in this case. Exactly what are those dots, and why do they move?
--Rik
But I am puzzled by what is physically going on in this case. Exactly what are those dots, and why do they move?
--Rik
Re: Zerene: How to stop the rotation of the stable subject
Hello Rik
But I couldn't explain it any other way .
It's quite funny if you scroll through the pictures quickly,
you can see the stationary hypostome and the passing dots.
Best,
ADi
That's just my guess, I don't have any hard evidence.the dots on the cover glass move
But I couldn't explain it any other way .
It's quite funny if you scroll through the pictures quickly,
you can see the stationary hypostome and the passing dots.
Best,
ADi
- rjlittlefield
- Site Admin
- Posts: 23625
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
Re: Zerene: How to stop the rotation of the stable subject
I have seen effects like this with bubbles in fluid. Were you looking through any fluid, or was everything solid?
--Rik
--Rik
Re: Zerene: How to stop the rotation of the stable subject
Hi Rik,
Preparation in Euparal, but no immersion oil; Nikon E Plan 100x/1,25
ADi
Preparation in Euparal, but no immersion oil; Nikon E Plan 100x/1,25
ADi
- rjlittlefield
- Site Admin
- Posts: 23625
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
Re: Zerene: How to stop the rotation of the stable subject
And the Euparal had hardened?
If so then this is a huge mystery!
Is the problem repeatable? Can you see it through the eyepieces (and distinguish it from floaters in your own eyes)?
--Rik
If so then this is a huge mystery!
Is the problem repeatable? Can you see it through the eyepieces (and distinguish it from floaters in your own eyes)?
--Rik
Re: Zerene: How to stop the rotation of the stable subject
Hi Rik,
This is a fresh and very thick preparation of a whole tick.
To stack the whole hypostome, the lens has pressed on the cover glass
so that the air bubbles in the euparal have shifted.
Best,
ADi
no, and that is also the explanation :-)And the Euparal had hardened?
This is a fresh and very thick preparation of a whole tick.
To stack the whole hypostome, the lens has pressed on the cover glass
so that the air bubbles in the euparal have shifted.
Best,
ADi
- rjlittlefield
- Site Admin
- Posts: 23625
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
- Contact:
Re: Zerene: How to stop the rotation of the stable subject
Excellent -- the mystery is solved!
--Rik
--Rik
- MarkSturtevant
- Posts: 1957
- Joined: Sat Nov 21, 2015 6:52 pm
- Location: Michigan, U.S.A.
- Contact:
Re: Zerene: How to stop the rotation of the stable subject
I wonder if there is a way to de-gas the mountant to remove air bubbles before it hardens. OR de-gas the mountant before its applied to the specimen?
I know putting the slides on a slide warmer accelerates the hardening, and this also helps to shrink bubbles but maybe not eliminate them.
I know putting the slides on a slide warmer accelerates the hardening, and this also helps to shrink bubbles but maybe not eliminate them.
Mark Sturtevant
Dept. of Still Waters
Dept. of Still Waters