I'm sure this has been answered before but i did'nt manage to find.
What i want is the mathematical formula for calculating the optimal step for a given objective (magnification and n/a) and sensor size.
Also a little theory regarding the logic of calculations will be very helping.
Thank you in advance.
Haris.A
Stacking step for microscope objective
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Google Nikon Microscopyu - don't forget the "u"!
This recent thread is relevant ...
http://www.photomacrography.net/forum/v ... hp?t=14527
This recent thread is relevant ...
http://www.photomacrography.net/forum/v ... hp?t=14527
- rjlittlefield
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Adding to Chris's comments...
I know two pages at microscopyu that are particularly relevant here.
http://www.microscopyu.com/articles/for ... depth.html has a general discussion, some formulas, and some examples.
http://www.microscopyu.com/tutorials/java/depthoffield/ has an interactive calculator that lets you plug in various parameters and see the calculated DOF that results.
Of the two, I'd put more faith in the calculator. Both pages show the same formula. The calculator tracks the formula exactly, but the values on the first page vary randomly -- sometimes low, sometimes high, by amounts that are large enough to matter. (I have no idea where the values come from. I asked the web site maintainers about this a few years ago, but never got an answer.)
Anyway, as noted in the thread that Chris linked, the calculations are only a rough guide to "optimal". Values from the calculator with minimum e should be safe for most purposes, but you should test in your own situation if it's important to be dead on.
--Rik
I know two pages at microscopyu that are particularly relevant here.
http://www.microscopyu.com/articles/for ... depth.html has a general discussion, some formulas, and some examples.
http://www.microscopyu.com/tutorials/java/depthoffield/ has an interactive calculator that lets you plug in various parameters and see the calculated DOF that results.
Of the two, I'd put more faith in the calculator. Both pages show the same formula. The calculator tracks the formula exactly, but the values on the first page vary randomly -- sometimes low, sometimes high, by amounts that are large enough to matter. (I have no idea where the values come from. I asked the web site maintainers about this a few years ago, but never got an answer.)
Anyway, as noted in the thread that Chris linked, the calculations are only a rough guide to "optimal". Values from the calculator with minimum e should be safe for most purposes, but you should test in your own situation if it's important to be dead on.
--Rik
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