100x obj. mag.

Bigger one here: https://flic.kr/p/2jXSjwy
Thanks!
Moderators: Pau, rjlittlefield, ChrisR, Chris S.
100x, epi-illumination, so it's very flat and even.Guppy wrote: ↑Mon Nov 02, 2020 8:16 amHi Dan
I really like your pictures.
Which 100x lens do you use?
Your pictures look a bit flat.
I assume that your lighting is all around and even, without shadows.
With lateral light you get soft shadows, the object gains depth and the visible resolution is higher.
Kurt
In principle, epi-illumination can be structured so that light comes out of just part of the front of the lens. If that part is sufficiently small and off-center, then you should get the corresponding modeling, complete with shadows.Macro_Cosmos wrote: ↑Mon Nov 02, 2020 10:14 am100x, epi-illumination, so it's very flat and even.
There's no other way to light it because of the short working distance.
In principle oblique illumination doesn't need to be difficult. If your epiilluminator allows it, you can put a mask close of the aperture diaphragm like with transmitted illumination. You could also decenter the lamp or the aperture diaphragm or both. Again like with transmitted, NA will be reduced to some extent if the objective rear aperture is not fully illuminated.rjlittlefield wrote: ↑Mon Nov 02, 2020 11:08 amIn principle, epi-illumination can be structured so that light comes out of just part of the front of the lens. If that part is sufficiently small and off-center, then you should get the corresponding modeling, complete with shadows.Macro_Cosmos wrote: ↑Mon Nov 02, 2020 10:14 am100x, epi-illumination, so it's very flat and even.
There's no other way to light it because of the short working distance.
But I have never tried to implement this, so I do not know what practical difficulties will turn up. I expect it will be quite difficult or impossible with many systems.
--Rik