Alright, alright, here is a question for the microscopy geeks among us. I have a Leitz Dialux II microscope, with a condenser that can't accept filters as-is, let alone phase contrast rings. But, I do want to add phase contrast. It's driving me crazy to not have it.
Based on previous experiments, I think it might be possible. Here is what I found:
- https://www.micromagus.net/microscopes/diyphase.html
- http://www.microscopy-uk.org.uk/mag/art ... phase.html
I have access to a 3D printer and a laser cutter, so I can manufacture or cut very precisely.
But I am new to microscopy, and lack some optical knowledge. That's where I need your help!
- In theory, if I design a filter holder that attaches to the bottom of the condenser, would it work ?
- If yes, how do I figure out the optimal dimensions for the phase rings ? Surely there must be a way without trial and error, which seems basically impossible due to the high number of combinations.
I got myself a Leitz 40x 160mm Phaco 2 objective to experiment.
Any pointer would be very much appreciated. Thank you!
PS Of course if I can get my hands on a suitable phase contrast condenser, that would be awesome, but, they seem unobtainium or those for sale are either overpriced or broken or miss parts...