Darkfield Adapter for Whole Slide Imaging

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Saul
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Darkfield Adapter for Whole Slide Imaging

Post by Saul »

Found time to play with the old project, which was inspired by:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592912/

Initial setup:

1.jpg
2.jpg
3.jpg
4.jpg
LEDs from the top & bottom are covered with the aluminum foill (to concentrate the light to the edge of the slide). Of course, there is a dimmer.
There is a slot in the front of LEDs to place filters (pol, color, etc)

First try:
https://www.photomacrography.net/forum/ ... 38#p264938

I liked the result, next step is to place more LEDs around the perimeter, in order to have more light control.

Anybody tried this type of illumination already ?
Saul
μ-stuff

Harald
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Re: Darkfield Adapter for Whole Slide Imaging

Post by Harald »

Hi Saul,
Looking forward to see some images with that kind of light.

Please share :D
Kind Regards
Harald

Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11

Saul
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Re: Darkfield Adapter for Whole Slide Imaging

Post by Saul »

3D model (alpha version):
viewtopic.php?p=265032#p265032
Saul
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Saul
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Re: Darkfield Adapter for Whole Slide Imaging

Post by Saul »

Beta version is coming - light from both sides (separate dimmers) & sample can be rotated.

IMG_1712-web600x800U100.jpg
Saul
μ-stuff

Smokedaddy
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Re: Darkfield Adapter for Whole Slide Imaging

Post by Smokedaddy »

... as always, very creative.

Saul
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Re: Darkfield Adapter for Whole Slide Imaging

Post by Saul »

Smokedaddy wrote:
Fri Jul 17, 2020 9:46 pm
... as always, very creative.
Thanks, James ! One of the next projects - time machine, in order to have more time to play with my toys ... ;)
Saul
μ-stuff

Harald
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Location: Steinberg, Norway
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Re: Darkfield Adapter for Whole Slide Imaging

Post by Harald »

Hi Saul,
This is just great.
It has given me some ideas.

Thank you so much for sharing this brilliant project :D
Kind Regards
Harald

Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11

Beatsy
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Re: Darkfield Adapter for Whole Slide Imaging

Post by Beatsy »

Saul wrote:
Fri Jul 17, 2020 8:59 pm
Beta version is coming - light from both sides (separate dimmers) & sample can be rotated.
Oh my! That could be so good for diatom arranging and easily adapted to my setup(s). I think I have some suitable parts to try it out too (but not LEDs and electronics :( ). I can probably simulate with fibre-optic goosenecks or LEDLenser torches for testing though. Great post that prompted quite a few related ideas for me. Thanks much!

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Scarodactyl
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Re: Darkfield Adapter for Whole Slide Imaging

Post by Scarodactyl »

This looks extremely rad.
There are fiber optic light guides that end in a flat bar rather than a rpund point. I wonder if these would be flat enough to use on a setup like this.

Pau
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Re: Darkfield Adapter for Whole Slide Imaging

Post by Pau »

Scarodactyl wrote:
Sat Jul 18, 2020 10:41 am
There are fiber optic light guides that end in a flat bar rather than a round point. I wonder if these would be flat enough to use on a setup like this.
Time go I did some tests with my dual gosseneck FO guide and it was very difficult to set up but worked well enough to prove the idea. I saw those flat bar FOs but they were too expensive for a device I really didn't need.
Pau

Saul
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Re: Darkfield Adapter for Whole Slide Imaging

Post by Saul »

Saul
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aidanmoore
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Re: Darkfield Adapter for Whole Slide Imaging

Post by aidanmoore »

This reminds me of the MVI Darklite I picked up on eBay a few years ago
dark1sc.jpg
It uses a halogen light source and a fiber optic tube, but the idea is the same
dark2sc.jpg
dark3sc.jpg
Here's some shots of some old Victorian mount slides using the Darklite:
treefixed_sc.jpg
geodesc.jpg
Nikon Eclipse 90i Blackmagic 4K studio camera Nikon D5300

dolmadis
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Re: Darkfield Adapter for Whole Slide Imaging

Post by dolmadis »

Saul wrote:
Wed Jul 15, 2020 5:57 pm
Anybody tried this type of illumination already ?
Hi Saul,

I found this link having Googled "MVI Darklight".

http://www.microbehunter.com/microscopy ... 6&start=60 

How do the microscope images turn out with your Version 1 and Version 2 which you labelled "Beta"?

Is the 3D file for Beta going to end up in The PMN 3D Archive?

This is a very interesting project and I have already sourced some diodes in the UK but you will have to be my consultant the power and dimmer details because like other Members it is not my forte (as you know !!).

Best

John

houstontx
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Re: Darkfield Adapter for Whole Slide Imaging

Post by houstontx »

I have a MVI Darklight as well. Need to drag it out of storage and use it a bit. Thanks for reminding me!

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