... and the reason I sent the video links to you. <g> You should send the Youtuber a message.mawyatt wrote: We have the same stages and using a 16 bit DAC at full scale for greater piezo stage resolution and range.
These are a special version of Nikon's similarly spec'd 20x NA0.75 objective which also requires a coverslip. They were used in a microscopy setup (TIRF) and spectral analysers. They can be found in the Illumina GAIIx gene sequencers (it utilises a TIRF microscope, 20x objective marked 0500-0087). The 1501-9398 is found in some kind of spectral analyser device thingy that I know little to nothing about, so I won't guess what it's supposed do do.physicsmajor wrote:Prior thread is very relevant https://www.photomacrography.net/forum/ ... 72&start=0
There are quite a lot of these Nikon 20x/0.75 OEM objectives out there right now, being sold as the OEM versions of the Plan Apo VC, and I don't think they were used in a microscope. One listing shows the lens mounted to an assembly very reminiscent of the photolithography setup shown in the original post (could also be for automated inspection, or gene sequencing as suggested in prior posts). With hundreds of objectives in a large order, Nikon might well have tweaked the lens for this order.
Also of interest, there may be two versions of this OEM objective, and the surplus dealers don't seem to have it completely straight. The YouTube poster has lens clearly marked 1501-9398 (see screenshot earlier in this thread). But the Ebay seller and listing pointed to by that YouTube poster shows pictures of an objective marked 0500-0087, which is also in the listing title. But in the text of that same listing it says "you will receive Nikon 20x Microscope Objective 1501-9398" - maybe they're the same, maybe not!
I don't suppose anyone has both...?
I don't think there is any reason to think this, especially since they are physically different. Different machines will have different wall thicknesses and may also use different wavelengths. One of these days I'll try to measure the optimum coverslip and degree of correction of my three versions (the two OEM versions and the real 20x VC)....I've been too busy to do so lately.These 2 should be optically identical.