40x LWD

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Harald
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40x LWD

Post by Harald »

Hi there good people,

Any recommendations for a good 40x objective LWD with RMS threads that doesn't cost an arm?
Kind Regards
Harald

Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11

Ichthyophthirius
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Post by Ichthyophthirius »

Hello Harald,

Any constraints?

- minimum NA?
- minimum working distance?
- use with eyepiece or direct projection?
- finite or infinite tube length?
- use with or without cover glass?

Regards, Ichty

Harald
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Joined: Fri May 13, 2011 10:33 am
Location: Steinberg, Norway
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Post by Harald »

I am going to use it on a trinocular bio microscope from AmScope to photograph, so I guess direct projection

Was hoping to get 2mm WD.
NA not critical and finite I think.

I am not good in these terms.

Shall use it to photograph micro minerals
Kind Regards
Harald

Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11

Lou Jost
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Post by Lou Jost »

Another important question- what size sensor?

Ichthyophthirius
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Post by Ichthyophthirius »

I can't think of many options. I had a look at your website; your setup seems to work well with Nikon objectives.

There are a few 40 LWD and ELWD from Nikon with 210 mm tube length and without cover glass. Some have > 2 mm free working distance. That would require a 50 mm intermediate tube being added to your microscope.

http://www.krebsmicro.com/mplan.pdf

Alternatively, a biological LWD objective with correction collar and the collar set to 0.

http://www.krebsmicro.com/Nikon_CF.pdf

enricosavazzi
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Post by enricosavazzi »

Ichthyophthirius wrote:[...]
There are a few 40 LWD and ELWD from Nikon with 210 mm tube length and without cover glass. Some have > 2 mm free working distance. That would require a 50 mm intermediate tube being added to your microscope.
[...]
The MPLAN ELWD 40x 0.50 in particular has slightly more than 10 mm working distance, so illumination of the subject is easier.

I found my specimen relatively good (that was years before we all started stacking, though), but of course not at the level of Mitutoyo M Plan Apos. The 0.50 NA is the limiting factor, plus some CA, and DOF is of course minuscule. It is somewhat tolerant of shorter tube length (and with the latter it gives a lower magnification and slightly higher working distance).

The only test I can find is at http://savazzi.net/photography/photomacrolenses2.htm
--ES

Harald
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Joined: Fri May 13, 2011 10:33 am
Location: Steinberg, Norway
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Post by Harald »

Hi,
Thanks for your time and great tips.

I will check them out
Another important question- what size sensor?
It will be a FF sensor on my EOS 5D II
The MPLAN ELWD 40x 0.50 in particular has slightly more than 10 mm working distance, so illumination of the subject is easier.
This is very interesting.
Edit: Found it on eBay for 305$
Kind Regards
Harald

Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11

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