Darkfield Microscopy ??

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marcojongsma
Posts: 15
Joined: Fri Feb 22, 2019 3:12 am

Darkfield Microscopy ??

Post by marcojongsma »

I have a question
I use microscope objectives mounted on bellows and a raynox 150 as a tube lens.
So no microscope.
Now I would also like to take darkfield photos is that possible with this setup? Do you have ideas how I can do this and what else I need to do so that I can record a water fly.
thanks in advance for your help

Regards marco

JohnyM
Posts: 463
Joined: Tue Dec 24, 2013 7:02 am

Post by JohnyM »

Sure. Light your subject from steep angle - like 90 degrees. And make sure your light wont reach the lens.

Photomicro
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Joined: Sat Aug 12, 2017 11:01 am
Location: UK

Post by Photomicro »

A ring light can achieve this quite well, if you get the distance from the subject right. Do this by trial and error.

This was done like this;

https://www.flickr.com/photos/66189529@ ... 783466119/
regards, Mike.

Time flies like an arrow, fruit flies like bananas.

https://www.flickr.com/photos/66189529@N08/

Chris S.
Site Admin
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Joined: Sun Apr 05, 2009 9:55 pm
Location: Ohio, USA

Post by Chris S. »

Marco, welcome to posting! :D

The advice you've gotten from Johnny and Mike is excellent. I'd add that getting a darkfield effect in an open macro system is pretty easy. As the others have said, experiment with light placement, but with the light never hitting the lens. You'll get it quickly.

--Chris S.

Pau
Site Admin
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Location: Valencia, Spain

Post by Pau »

What magnification and -more important- NA do you plan to use for dark field?

With high NA could be easier to use a microscope dark field condenser.
Pau

Alan Wood
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Joined: Wed Dec 29, 2010 3:09 pm
Location: Near London, U.K.
Contact:

Post by Alan Wood »

Here is one way of achieving dark-ground illumination without a microscope:

Image

Inside the 144 LED ring-light is a disk of black card or velvet.

The specimen (a slide in this case) rests on a piece of black foam board with a circular or rectangular hole.

The foam board is supported by a kitchen bowl with its base removed.

Here is a photograph taken with this arrangement:

Image

Alan Wood

marcojongsma
Posts: 15
Joined: Fri Feb 22, 2019 3:12 am

Post by marcojongsma »

Pau wrote:What magnification and -more important- NA do you plan to use for dark field?

With high NA could be easier to use a microscope dark field condenser.

5x and 10x magnification i use olympus uplan fln 0.30

marcojongsma
Posts: 15
Joined: Fri Feb 22, 2019 3:12 am

Post by marcojongsma »

Photomicro wrote:A ring light can achieve this quite well, if you get the distance from the subject right. Do this by trial and error.

This was done like this;

https://www.flickr.com/photos/66189529@ ... 783466119/
Thank you for the tips

marcojongsma
Posts: 15
Joined: Fri Feb 22, 2019 3:12 am

Post by marcojongsma »

Alan Wood wrote:Here is one way of achieving dark-ground illumination without a microscope:

Image

Inside the 144 LED ring-light is a disk of black card or velvet.

The specimen (a slide in this case) rests on a piece of black foam board with a circular or rectangular hole.

The foam board is supported by a kitchen bowl with its base removed.

Here is a photograph taken with this arrangement:

Image

Alan Wood
Thank you for the tips and photos i think im gonna build someting similair

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