This link that i already posted:By the way, if you find useful references such as lists of old cubes/filters/handy fluorophores or equipment documents, I'll put a link in the original post. Send a pm if you prefer.
https://www.microscopyu.com/techniques/ ... ilter-sets
Includes all legacy Nikon cubes, as they havent changed much/ at all (except multibands).
@Pau quite a task you gave me. Allow me to answer in parts.
I do not have any resource, althrough i do have acces to Colibri (atm it's 300km away, so cant do a test) but they are very weak indeed. Weaker than mine (referenced further in this post). Althrough Zeiss setup provide EXCELLENT pure black background.Pau wrote: Do you know the emitting power of Colibri LEDs?
... I have the impression that they are low powered
I've seen quite a few damaged too. From my experience it's due to cleaning... with shirt etc. Scope have 4 holes for cubes (some newer scopes accept 6, im working on mod for 8 atm). We got like 20 cubes. In storage, they got dusty / fingerpints when swapping them. Since there are many users with many needs, and none of them ever read the manual... they often attempt to quickly "fix" their error of touching the filter and ruining it in the process.Pau wrote: I've seen lots of filters heavily damaged, mainly excitation ones, although not knowing their history, likely they came from labs and had been in not careful continuous daily use for many years, a very different situation of amateur use.
My led setup:Pau wrote: At least for UV I don't think that halogen could compete at all with modern LEDs (if so it would be extremely dangerous!)...Again, could you kindly provide exposure data of an example?-Power, it's no competition. Imagine using F5.6 zoom in a church. Then switch it to F1.4 prime. It's something like that.
All leds are 3W "high power" 365, 405, 455 nm
They were mounted in place of mirror in HBO lamphouse.
Nikon CFN PlanFluor 10x/0.3 through 1,5x teleconverter on A6300. ISO set on 100, camera in A-mode will chose shutter according to its whim.
Target is low photobleaching, high autofluorescence... office paper:
Now the comparision:
-First row is LED
-Second row is 12V100W halogen
-3rd row is 100W HBO mercury burner
1st column is UV 365nm (UV-2A)
2nd column is V 405nm (V-1A)
3rd column is B 470nm (B-2A)
Full res screen capture montage:
https://drive.google.com/open?id=19N5dW ... Rm1526DOyD
Ok i admit, i was wrong. Difference i've mentioned is between Halogen and LED. HBO is like power of sun at your fingertips...
-2,66 stops advantage over 3W led in UV
-2,33 stops advantage over 3W led in V
-2,33 stops advantage over 3W led in B
-7,66 stops advantage over 100w halogen in UV
-6 stops advantage over 100w halogen in V
-1 stop advantage over 100w halogen in B
(in green light, halogen takes advantage over HBO btw)
-11 stops advantage over 3W led in UV
-8,33 stops advantage over 3W led in V
-3,66 stops advantage over 3W led in B
Differences might be slightly larger/smaller as i didnt payed much attention to focusing / centering.