"OEM Nikon 20x 0.75 VC" cover slip thickness
Moderators: rjlittlefield, ChrisR, Chris S., Pau
you are right. my bad.Lou Jost wrote:Munich, I recognize those figures from the patent application referenced in our original thread...I imagine the text is just a Japanese translation of the English in that application?
https://patents.google.com/patent/US8143599
Pau, as I understood it, that was for an implementation which included the option to focus on either the upper or lower inner surface of the flow cell. Abednego shows that the actual implementation only focuses on the lower inner surface, so no compensator would be needed because the objective would be optimized to image that surface.
I know this doesn't answer your actual question, but earlier it was suggested that this compensator would be a weak negative lens element.
I know this doesn't answer your actual question, but earlier it was suggested that this compensator would be a weak negative lens element.
Yes, I recall it, although it seemed just a guess. It it were a weak negative lens it would not project the image to infinite but "past infinite". If so, it could be done focusing the tube lens itself past infinite, just placing it nearer of the the sensorLou Jost wrote:I know this doesn't answer your actual question, but earlier it was suggested that this compensator would be a weak negative lens element.
I tried to discuss it at
http://www.photomacrography.net/forum/v ... 901#222901
Pau
Yes, though chances are that you'd get 500 identical!If accuracy is not important for that application, that could be a good thing.
The cytometer just needs a heavy cover, to sit firmly and not bend with "capillary suction" while cells are counted. But not another microscope slide because that would hit the objective.
Last edited by ChrisR on Tue Jan 01, 2019 11:10 am, edited 1 time in total.
Chris R
-
- Posts: 84
- Joined: Tue Nov 01, 2016 11:53 pm
There's an unimplemented Nikon patent application that describes a spherical aberration compensator for both finite and infinite systems.
The embodiment in fig.3 is actually a representation of a 20x NA0.75 objective and a compensator...coincidence?
https://patents.google.com/patent/US597 ... tor&page=9
The embodiment in fig.3 is actually a representation of a 20x NA0.75 objective and a compensator...coincidence?
https://patents.google.com/patent/US597 ... tor&page=9
What about these???? Aberration correcting plates:
https://www.edmundoptics.com/f/spherica ... ates/14001
https://www.edmundoptics.es/resources/a ... on-plates/
A bit on the expensive side. Maybe there are cheaper ones somewhere?
https://www.edmundoptics.com/f/spherica ... ates/14001
https://www.edmundoptics.es/resources/a ... on-plates/
A bit on the expensive side. Maybe there are cheaper ones somewhere?
From Edmund's info:Lou Jost wrote:What about these???? Aberration correcting plates:
https://www.edmundoptics.com/f/spherica ... ates/14001
https://www.edmundoptics.es/resources/a ... on-plates/
A bit on the expensive side. Maybe there are cheaper ones somewhere?
They are excellent for correcting spherical aberrations in systems that have small fields of view.
Pau
"1501-9398 and 0500-0087 are most likley the same, but with different inventory numbers by illumina for different machines."
Re-opening this thread because I have these two objectives in front of me now, along with a real Nikon 20x 0.75 VC objective.
The 0500-0087 is different from the other two, in that it has fat white seals:
These fat white seals suggest that this version of the 20x objective is meant for immersion. The Nikon 60x VC oil immersion objective has these seals, for example:
https://www.ebay.com/p/1304654369
On the other hand, I found an example in the scientific literature of someone using this objective in air to focus a laser beam, and calling it an "air" objective; but their name for the objective looked like it was copied from an ebay ad, so may not be reliable.
Here's a water immersion objective with similar seals:
https://www.ebay.com/itm/NIKON-CFI-Plan ... SwhJteDP3g
Is it possible that this 0500-0087 version of the 20x 0.75 objective is meant to be used with a drop of oil or water between it and the flow chamber?
Re-opening this thread because I have these two objectives in front of me now, along with a real Nikon 20x 0.75 VC objective.
The 0500-0087 is different from the other two, in that it has fat white seals:
These fat white seals suggest that this version of the 20x objective is meant for immersion. The Nikon 60x VC oil immersion objective has these seals, for example:
https://www.ebay.com/p/1304654369
On the other hand, I found an example in the scientific literature of someone using this objective in air to focus a laser beam, and calling it an "air" objective; but their name for the objective looked like it was copied from an ebay ad, so may not be reliable.
Here's a water immersion objective with similar seals:
https://www.ebay.com/itm/NIKON-CFI-Plan ... SwhJteDP3g
Is it possible that this 0500-0087 version of the 20x 0.75 objective is meant to be used with a drop of oil or water between it and the flow chamber?
Hmm, I see that my Nikon 20x that I sold to ChrisR last year has the same fat white seal...yet that lens is clearly made to be used in air.
https://www.photomacrography.net/forum/ ... highlight=
https://www.photomacrography.net/forum/ ... highlight=
-
- Posts: 1619
- Joined: Sat Apr 14, 2018 10:26 am
I am really curious if the two different unbranded Nikons will perform the same. I love my branded 20x and the 0500-0087 performs similarly though not quite as easy for my application (but I still need to test with an appropriate tube lens). If their 1501 is different I'd love to add one to the lineup.