Gradient Universal Filter - 'DIC', DF, OL effects

Every 30 days the site administrators will pick an image made through a microscope from the "Photography Through the Microscope Gallery" to be featured on the front page of the www.amateurmicrography.net website.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

Mitch640
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Post by Mitch640 »

Ah, OK, thanks. I missed that cause I was looking at the great images. :)

Charles Krebs
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Post by Charles Krebs »

Litonotus,

It works beautifully! Thanks for giving us a look at both the images and your custom filter.

Jacek
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Post by Jacek »

These are my photos taken through the filter Litonotus friend
Image
Image

Jan l'Amie
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Post by Jan l'Amie »

I just made your universal filter.
The first photo I made with my moonshaped blue stop filter.
The second is made with your filter.
This is just a quick one, I'm sure it can be much better.
Your filter works great. Thanks

Greetings
Jan

Image

Image
Byomic BYO500T microscope /CIOC XDS-1 invert.
Byomic ST-340 stereomicroscope
Olympus BHM Metallurgical Microscope
Canon EOS 7D Mark II / Canon MP-E65
Cognisys StackShot
www.ngvm.nl

Litonotus
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Post by Litonotus »

thank you all (:

looks good (: grey background is great for observations, blue just looks better, and is sharper here (I think it is not because the filter). lucky you, I haven't seen the micrasterias yet /:

Jacek - great (:

Jan l'Amie
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Post by Jan l'Amie »

Lots of Micrasterias thomasiana, rotata, crux-melitensis and truncata in a pond about 15 km from my house.
Byomic BYO500T microscope /CIOC XDS-1 invert.
Byomic ST-340 stereomicroscope
Olympus BHM Metallurgical Microscope
Canon EOS 7D Mark II / Canon MP-E65
Cognisys StackShot
www.ngvm.nl

Litonotus
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Post by Litonotus »

Jan l'Amie wrote:Lots of Micrasterias thomasiana, rotata, crux-melitensis and truncata in a pond about 15 km from my house.
and about 1500 km from mine ;)

Pau
Site Admin
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Location: Valencia, Spain

Post by Pau »

Jacek wrote:These are my photos taken through the filter Litonotus friend
...
Excellent. At the posted size I don't see any disadvantage respect true DIC (altough I'm a DIC beginner)

If there was in the forum any kind of award for "the best DIY device" it must be for Litonotus
Pau

Litonotus
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Post by Litonotus »

thank you, Pau; but I think the best award for me is satisfaction that I invented something new (:

one image of a larva, taken using the blue filter

Image

and a copepod (grey filter)

Image

RogelioMoreno
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Post by RogelioMoreno »

Litonotus,

Excellent!

Are you buying your Nikon DIC setup to complement it? It looks like you do not need DIC.

Rogelio

Litonotus
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Post by Litonotus »

thank you (:

I'm trying to complete it, and someday it would be perfect to compare results. It is hard to imagine that this filter could be better than Nikon system... who knows? ;)

My filter works great on lower magnifications, and when the specimen is big, for ex. water fleas, copepods, larvaes, big algaes etc. You can't get the same effect on 40x objective and higher /:

Jan l'Amie
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Post by Jan l'Amie »

Great pictures.
I read somewhere that this not will work if the NA factor of your objective is higher than 0.65
This filter works great and I think it is a little cheaper than the Nikon stuff. :?
Byomic BYO500T microscope /CIOC XDS-1 invert.
Byomic ST-340 stereomicroscope
Olympus BHM Metallurgical Microscope
Canon EOS 7D Mark II / Canon MP-E65
Cognisys StackShot
www.ngvm.nl

Litonotus
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Post by Litonotus »

Jan l'Amie wrote: I read somewhere that this not will work if the NA factor of your objective is higher than 0.65
it works but gives only 'traditional' obligue light, it works better on 100/1,25 than on 40/0,65. filters worked great on a nikon 40/0,55 LWD objective.

I will post an image taken on a 100x objective.
Jan l'Amie wrote:This filter works great and I think it is a little cheaper than the Nikon stuff. :?
yes, only a little ;)

Litonotus
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Post by Litonotus »

As I said I'm posting two images from high magnifications.

here is a very old image (meridion circulare), I do not remember filter I used, but it was before I invented my universal gradient filter. it is quite good anyway and confirms that assumption with high NA. that nikon LWD objective had NA 0,55, I regret that I sold it but I had to save for other microscope items I'm collecting.
Image

pediastrum boryanum - universal filter on 100/1,25 - this is the best I can get, with PS postediting of course. it gives only obligue light here.
Image

Jacek
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Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

Difflugia sp filter Litonotus

Image

http://www.youtube.com/watch?v=0tlvWcEcyDs

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