Vitamin C crystal in polarized light

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Harald
Posts: 617
Joined: Fri May 13, 2011 10:33 am
Location: Steinberg, Norway
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Vitamin C crystal in polarized light

Post by Harald »

Hi there,
This is my result from a weekend of experimenting. This is a single shot, no stacking. Magnification is about 150x. Canon Eos 7D.

Image

Hope you like it :shock:
Kind Regards
Harald

Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11

RogelioMoreno
Posts: 2962
Joined: Fri Nov 20, 2009 11:24 am
Location: Panama

Post by RogelioMoreno »

Very nice!

How did you prepare the slide?

Rogelio

Harald
Posts: 617
Joined: Fri May 13, 2011 10:33 am
Location: Steinberg, Norway
Contact:

Post by Harald »

As I´ve learned C-Vitamin is the same as Acidum Ascorbicum. This was bought in the local pharmacy and dissolved in the ratio 1:50 with water. I put one drop on the microscope slide and waited for about one day for the crystals to form. Water evaporates and the crystals remain on the slide. The most exciting thing is that you never know what you get. You can get 100´s of images on just one slide. Try different magnifications.
I hope this helps and feel free to experiment :P
Kind Regards
Harald

Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11

bromodomain
Posts: 155
Joined: Sun Jan 02, 2011 11:50 am

Post by bromodomain »

I have some lab grade ascorbic acid that I have never looked at and this thread inspired me to take a few shots.

BTW what is your microscope? What polarizers does it have?

Harald
Posts: 617
Joined: Fri May 13, 2011 10:33 am
Location: Steinberg, Norway
Contact:

Post by Harald »

Hi,
I use the Amscope T230A and in this shot I used an 10x Plan objective. I also use a third part camera adapter with my Canon 7D.
The polarizer filter I´ve used is Linear Pola from Hoya. Took one apart and fitted inside the camera adapter and one over the light source, works just fine. As I can turn the filter over the light I have full control..
Kind Regards
Harald

Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11

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