An aster floret cross sections

Images made through a microscope. All subject types.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

Jean-marc
Posts: 283
Joined: Sat Oct 06, 2007 2:57 pm
Location: France
Contact:

Re: An aster floret cross sections

Post by Jean-marc »

Best cross section image I have ever seen ! =D> =D>

Olympusman
Posts: 5090
Joined: Sun Jan 15, 2012 12:31 pm

Re: An aster floret cross sections

Post by Olympusman »

ZOWIE!

Mike
Michael Reese Much FRMS EMS Bethlehem, Pennsylvania, USA

Bob-O-Rama
Posts: 200
Joined: Sat Dec 06, 2014 6:46 am
Location: Allentown, PA, USA, Earth, etc.
Contact:

Re: An aster floret cross sections

Post by Bob-O-Rama »

That's just amazingly beautiful work, and great info on your technique.

bobfriedman
Posts: 275
Joined: Tue Jul 28, 2009 6:02 pm
Contact:

Re: An aster floret cross sections

Post by bobfriedman »

another wow!... excellent rendering.

pierre
Posts: 288
Joined: Mon Jan 04, 2010 12:37 pm
Location: France, Var, Toulon

Re: An aster floret cross sections

Post by pierre »

Impressive work !
Regards

Pierre

Harald
Posts: 678
Joined: Fri May 13, 2011 10:33 am
Location: Steinberg, Norway
Contact:

Re: An aster floret cross sections

Post by Harald »

That calcofluor doesn't come cheap! :?
But I guess you don't have to use much of it? :-k
Kind Regards
Harald

Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11

bobfriedman
Posts: 275
Joined: Tue Jul 28, 2009 6:02 pm
Contact:

Re: An aster floret cross sections

Post by bobfriedman »

very nice work... congrats!

Lou Jost
Posts: 5942
Joined: Fri Sep 04, 2015 7:03 am
Location: Ecuador
Contact:

Re: An aster floret cross sections

Post by Lou Jost »

Really stunning! Can you tell us something about the optics and lighting?
Also, pardon my ignorance, but what is PBS and RT?

blepharopsis
Posts: 188
Joined: Fri Nov 28, 2014 12:39 pm
Location: Virginia, USA
Contact:

Re: An aster floret cross sections

Post by blepharopsis »

Thank you!
the images were taken with a fluorescent microscope using lasers as a source of excitatory light - you may find more info on confocal microscopy here: https://en.wikipedia.org/wiki/Confocal_microscopy .

PBS = phosphate-buffered saline; a solution of salt (sodium chloride) at physiological concentration (~0.9%), buffered (Ph-stabilized) with sodium and potassium phosphates.
RT = room temperature (20 degrees Celsius, 68 F).


Lou Jost wrote:
Sat Feb 04, 2023 3:15 pm
Really stunning! Can you tell us something about the optics and lighting?
Also, pardon my ignorance, but what is PBS and RT?

Lou Jost
Posts: 5942
Joined: Fri Sep 04, 2015 7:03 am
Location: Ecuador
Contact:

Re: An aster floret cross sections

Post by Lou Jost »

Thank you. Ii didn't realize they were done with a confocal microscope.

Marek Mis
Posts: 2586
Joined: Sat Jul 10, 2010 9:56 am
Location: Suwalki, Poland
Contact:

Re: An aster floret cross sections

Post by Marek Mis »

Igor, your confocal images are from totally different world ! Just incredible !

blepharopsis
Posts: 188
Joined: Fri Nov 28, 2014 12:39 pm
Location: Virginia, USA
Contact:

Re: An aster floret cross sections

Post by blepharopsis »

Thank you, Marek! You are a master of capturing micro alien worlds, though :)

Marek Mis wrote:
Wed Apr 05, 2023 1:49 am
Igor, your confocal images are from totally different world ! Just incredible !

Sippyjug
Posts: 65
Joined: Mon Nov 19, 2018 9:29 am

Re: An aster floret cross sections

Post by Sippyjug »

Absolutely stunning. Worthy of a standing ovation. =D>

lothman
Posts: 956
Joined: Sat Feb 14, 2009 7:00 am
Location: Stuttgart/Germany

Re: An aster floret cross sections

Post by lothman »

=D> =D> =D> =D>
I rarely look in the microscope forum, but now I'm glad I did. Thanks for sharing your technique and those stunning pictures.

Post Reply Previous topicNext topic