Rosa blanda Rhizome with Starch

Images made through a microscope. All subject types.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

ldflan
Posts: 71
Joined: Sat Mar 11, 2017 5:05 pm
Location: Issaquah, WA

Rosa blanda Rhizome with Starch

Post by ldflan »

A single Nomarski prism acts (I think) as about a 1/16 wavelength retarder plate. Using just one Nomarski prism and rotating your polarizers to suit can be useful for giving a slight birefringence effect without too-radically altering the apparent colors of your sample. Here for example is a picture of starch granules undergoing enzymatic digestion in the outer cortex of a Rosa blanda rhizome. (Fiixed in FAA, sectioned on a vibratome at about about 30um thick, cleared in Visikol for Plant Biology TM, no stain, imaged at 400x on a Nikon Diaphot TMD using a 40x DIC objective and crossed polarizers with a single Nomarski prism in place behind the objective.) The "maltese cross" interference pattern that you get with starch granules imaged under crossed polarizers is apparent here, but not too striking.
R blanda Rhizome.jpg
Leonard

Post Reply Previous topicNext topic