Seaweeds fluorescence
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Seaweeds fluorescence
Few algae autofluorescence stacks
Samples collected few days ago at the Mediterranean rocky shore (Xabia), less than 1m deep.
Both under UV and blue excitation brown and red algae produce the typical red chlorophyll emission while red algae produce very strong yellow emission.
Dictyota sp (Phaeophyta) tallus with unidentified red algae growing on it. UV 365nm LED epifluorescence
Corallina sp (Rhodophyta), blue 480nm LED epifluorescence
Unidentified filamentous green alga (Chlorophyta). UV 365nm LED epifluorescence
All taken with Leitz PL Fluotar 10/0.30, custom LED illuminatation on Zeiss WL. EOS 7D afocal 1.6X
Samples collected few days ago at the Mediterranean rocky shore (Xabia), less than 1m deep.
Both under UV and blue excitation brown and red algae produce the typical red chlorophyll emission while red algae produce very strong yellow emission.
Dictyota sp (Phaeophyta) tallus with unidentified red algae growing on it. UV 365nm LED epifluorescence
Corallina sp (Rhodophyta), blue 480nm LED epifluorescence
Unidentified filamentous green alga (Chlorophyta). UV 365nm LED epifluorescence
All taken with Leitz PL Fluotar 10/0.30, custom LED illuminatation on Zeiss WL. EOS 7D afocal 1.6X
Pau
Re: Seaweeds fluorescence
Beautiful. How are you dealing with quenching and bleaching?
Re: Seaweeds fluorescence
Thanks ADi, Luis Carlos and Lou!
In this cases algae bleaching was somewhat slower than with the typical plants like ferns, in special the Phaeophyta Dictyota
I've shot with the camera in AV mode and corrected contrast and exposure in PP, also I've enabled exposure correction in Zerene, so the images are pretty heavily processed (also lots of dead pixel erasing and strong sharpening)
In the Rhodophyta yellow emission bleaching is negligible, at least during a 10min set up and stack session, but as you well know chlorophyll photobleaching is important and annoying.How are you dealing with quenching and bleaching?
In this cases algae bleaching was somewhat slower than with the typical plants like ferns, in special the Phaeophyta Dictyota
I've shot with the camera in AV mode and corrected contrast and exposure in PP, also I've enabled exposure correction in Zerene, so the images are pretty heavily processed (also lots of dead pixel erasing and strong sharpening)
Pau
Re: Seaweeds fluorescence
Acetabularia mediterranea , for me the most beautiful alga, an umbrella shaped giant unicellular Chlorophyta alga measuring up to several centimeters. This one is a small sprcimen, about 7mm diameter.
UV 365nm with external torch, old Leitz Plan 2.5/0.08, short stack
UV 365nm with external torch, old Leitz Plan 2.5/0.08, short stack
Pau
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Re: Seaweeds fluorescence
Last one in the first post is my favourite, look at the colours, it's a bit... "hellish?", maybe "gothic" as well, amazing.
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Re: Seaweeds fluorescence
Such beautiful colors. Thank you.
Re: Seaweeds fluorescence
Nice work. Thanks for showing.
Re: Seaweeds fluorescence
Thanks Macro_Cosmos, Didier_Micro and ModelZ for your comments.
I didn't see that humble alga diabolic until your comment
I didn't see that humble alga diabolic until your comment
Pau
Re: Seaweeds fluorescence
Amazing images!
Zeiss Standard WL & Wild M8
Olympus E-p2 (Micro Four Thirds Camera)
Olympus E-p2 (Micro Four Thirds Camera)
Re: Seaweeds fluorescence
Very nice!
Dave
Dave
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Re: Seaweeds fluorescence
Beautiful photos nice work.
RB
RB