Hi,
In the last few weeks I could not expend much time with scope and when I did I did not manage to get results worth sharing.
A new stack, adapting to the microscope is proving to be quite challenging. Preparing the samples and working with live moving subjects.
It took me several times until I could complete a stack of this Diatom (ID?) from a marine water sample, it reacted a little to polarized light so I run this one. 22 shots with nikon CFN Planapo 40/0.95 160/0.17; direct projection plus 50% crop with Sony A5100
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Until now with every diatom (or almost anything else) I have tried to run a stack with I had to retouch from individual frames, no way I can get a clean stack straight on. Deciding what details to take from each frame is difficult as I do not fully understand yet what I am really looking at and which details are important to keep.
Also a short BF video I made
One more; this time BF with same lens, stack from 21 images
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A different polarized stack, this time I left the backgound blue, which seems to suit it better than trying to fix the WB
Stack from 18 images, same objective.
I made two versions; first like before showing internal details
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and one trying to keep only superficial texture, more translucent like. There seems to be some banding, will try to fix it
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Polarized live Diatom (Pleurosigma sp)
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Polarized live Diatom (Pleurosigma sp)
Last edited by seta666 on Mon May 10, 2021 6:55 am, edited 4 times in total.
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Re: Polarized live Diatom
Well, painful as the workflow might be the results are hard to argue with. Great work!
Re: Polarized live Diatom (Pleurosigma sp)
Thanks!! Glad you like it.
Seems like a pleurosigma indeed, forgot to mention it is from a marine water sample
Seems like a pleurosigma indeed, forgot to mention it is from a marine water sample
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Re: Polarized live Diatom (Pleurosigma sp)
It's a resolution chart! The small pores pose a major challenge, the are hard to resolve.