Polished section.
Episcopic illumination (Nikon Optiphot, Nikon BD Plan 50x 0.85 210/0, Canon 500D)
*ORIGINAL_RESOLUTION*
SEM, BSE
*ORIGINAL_RESOLUTION*
SEM, EDS PHASE MAP
*ORIGINAL_RESOLUTION*
The First image is a stack of ~20 photos, there appears to be some detail loss (and artifacts), in comparison to single images, when stacking such thin transparent objects, like crystals (Ca3SiO5*Ca2SiO4, yellow zones).
I wonder, maybe there is a way to get around this issue. I've tried to retouch it manually, but with little to no luck.
Portland cement particle
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Portland cement particle
“Thoroughly conscious ignorance is the prelude to every real advance in science.” - JCM
Re: Portland cement particle
Few more particles, for good measure. Episcopic illumination (Nikon Optiphot, Nikon BD Plan 50x 0.85 210/0, Canon 500D)
*ORIGINAL_RESOLUTION*
*ORIGINAL_RESOLUTION*
This "rainbow" interference effects happen only on transparent objects, while opaque objects perform more or less stable even in deep diffraction territory.
Gold sheets in Pt-Pd selenates. Episcopic illumination (Nikon Optiphot, Nikon PlanAPO 150x 0.95 210/0, Canon 500D) *ORIGINAL_RESOLUTION* *ORIGINAL_RESOLUTION*
^^SEM, BSE
This "rainbow" interference effects happen only on transparent objects, while opaque objects perform more or less stable even in deep diffraction territory.
Gold sheets in Pt-Pd selenates. Episcopic illumination (Nikon Optiphot, Nikon PlanAPO 150x 0.95 210/0, Canon 500D) *ORIGINAL_RESOLUTION* *ORIGINAL_RESOLUTION*
^^SEM, BSE
“Thoroughly conscious ignorance is the prelude to every real advance in science.” - JCM
Re: Portland cement particle
Itr is really interesting to see the visible-light image and the SEM of exactly the same thing at the same magnification. I don't think I have ever seen such a pair before.
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Re: Portland cement particle
I have studied the full resolution image, but I cannot identify "detail loss (and artifacts)" in the stacked image alone. Probably I would need to see a single image also, to understand the specific problems you are encountering.Duke wrote: ↑Tue Mar 30, 2021 5:52 amThe First image is a stack of ~20 photos, there appears to be some detail loss (and artifacts), in comparison to single images, when stacking such thin transparent objects, like crystals (Ca3SiO5*Ca2SiO4, yellow zones).
I wonder, maybe there is a way to get around this issue. I've tried to retouch it manually, but with little to no luck.
As a general comment, it is common that stacks shot at large NA somehow look "messy" compared to single images. Often this is due to the "squirming around" effect illustrated at https://www.photomacrography.net/forum/ ... 87#p149187 . The problem is tough enough with opaque subjects. It may be even worse with transparent subjects, and I do not know any great way to get around the issue. Whenever a problem cannot be fixed by manual retouching, that is a good indication that it's a hard problem!
I agree with Lou, by the way. It is very interesting to see side-by-side optical and SEM images of the same subject at the same magnification. Thanks for the comparison.
--Rik