Stereo Ostracods

Images made through a microscope. All subject types.

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micro_pix
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Stereo Ostracods

Post by micro_pix »

A couple of cross-eyed stereo pairs of empty Ostracod shells from a nearby beach.

Dave
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ostroshell33.jpg
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ostroshell34.jpg

Pau
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Re: Stereo Ostracods

Post by Pau »

Wow!

Excellent images.

Could you kindly provide some details? (illumination, magnification...)
Pau

rjlittlefield
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Re: Stereo Ostracods

Post by rjlittlefield »

Nice images!

I like the stereo in the first one. That pair is particularly interesting when I pull it into StereoPhoto Maker and zoom in. Some of the details reveal obvious thickness of the shell, independent of its overall convex shape.

The second one does not have nearly enough separation for my eyes. I can see a bit of rotation between the left/right views when I pull the pair into StereoPhoto Maker, shift the two halves into alignment, and repeatedly click the Swap Sides button. But in a static view, I mostly get the impression of "flat".

Do you know what the angular separation is, in each pair? Alternatively, do you know the field width, step size, number of frames, and shift % ? The angular separation can be calculated from those, using the formulas at https://zerenesystems.com/cms/stacker/d ... wing_angle .

--Rik

micro_pix
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Re: Stereo Ostracods

Post by micro_pix »

Thanks, Pau and Rik.

I agree Rik the second one looks flat. I’m pretty sure that I didn't alter the settings between those two pairs I''ll have a look.

I was using an Olympus LMPlan FL 20x and Raynox 5320 pro as a tube lens, on a vertical set-up which is a BH2 MJL block and 8" x 8" stage, bolted to a Thor Labs breadboard and sat on a Kaiser copy stand. The MJL focus is driven by a Wemacro micromate. The camera was a Canon M50 MkII and it was lit by two heavily diffused Yongnuo 560 IV Speedlites.

Here is a stereo foram shell from the same sample, this is around 0.6mm in diameter.

Dave
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2021-03-19-17.03.jpg
Last edited by micro_pix on Fri Mar 19, 2021 12:10 pm, edited 1 time in total.

micro_pix
Posts: 468
Joined: Fri May 11, 2012 12:05 pm
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Re: Stereo Ostracods

Post by micro_pix »

I re-ran the second one with more separation.

Dave
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2021-03-19-23.31.jpg

rjlittlefield
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Re: Stereo Ostracods

Post by rjlittlefield »

This has a lot more separation. But I notice that it also has some vertical disparities. The left-side image is a little higher than the right, and the rotation axis appears to be more or less aligned with the axis of the ostracod instead of being vertical as it should be. Did you rotate the images after rendering, or use both X and Y shifts simultaneously?

--Rik

micro_pix
Posts: 468
Joined: Fri May 11, 2012 12:05 pm
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Re: Stereo Ostracods

Post by micro_pix »

Thanks Rik! Yes I think I must have had an X and Y shift parameter in there, well spotted! I’ll run it again.

Dave

micro_pix
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Re: Stereo Ostracods

Post by micro_pix »

I think I've got the right settings now. Here are another two shells.
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2021-03-20-20.08.jpg
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2021-03-20-15.09.jpg
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Pieces of the following tubular structures are common in the sand samples that contain the Forams and Ostracod shells. It's fragile/brittle and I assume it's calcium carbonate or silica based and has an intricate lattice. The width is around 140 microns and broken pieces are 1-1.5mm long. If anyone could tell me what it is I'd appreciate it.
Edit: Some sea urchin spines have a very similar structure to this, I did think it was too small to be a sea urchin spine fragment but maybe that's what it is.
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2021-03-20-18.08.jpg
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2021-03-20-17.50.jpg
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A stereo view
2021-03-20-17.58.jpg
Dave
Last edited by micro_pix on Tue Jun 22, 2021 2:54 pm, edited 3 times in total.

rjlittlefield
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Re: Stereo Ostracods

Post by rjlittlefield »

Bingo! Stereo looks great now. Nicely done.

--Rik

micro_pix
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Re: Stereo Ostracods

Post by micro_pix »

Thanks for the input Rik.

Here are two more stereos.

Dave

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2021-03-21-23.19.jpg
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2021-03-21-23.33.jpg

rjlittlefield
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Re: Stereo Ostracods

Post by rjlittlefield »

Lovely stuff! Even with the excellent modeling light, I see aspects of the 3D structure in stereo that I cannot see in any single view.

--Rik

micro_pix
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Re: Stereo Ostracods

Post by micro_pix »

Thanks Rik.

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