Diatoms DIC

Images made through a microscope. All subject types.

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Macro_Cosmos
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Diatoms DIC

Post by Macro_Cosmos »

First DIC attempt. 40x UIS objective with the U-DIC40 UIS2 prism. Does it look good enough? Hopefully purchasing a UIS2 40x is no longer necessary.
Diatoms from Oamaru, NZ.
Image

I adjusted the prism to get a neutral background colour. The U-DICT can be adjusted to create all kinds of interesting colour casts. :D
100x up next, I only have 2 UIS 100x objectives. Hopefully they work with this UIS2 prism.

Macro_Cosmos
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Location: Sydney

Re: Diatoms DIC

Post by Macro_Cosmos »

Image
100x, +500 (!!!) sharpening in Capture One!
I am surprised and pleased with the result, even though there's prism mismatch going on.

100%: https://live.staticflickr.com/65535/503 ... 905b_o.jpg

Cute little thing, looks like a fidget spinner. Some tearing can be seen on the bottom left, I guess that's where the needle went for micromanipulation. :shock:
It can be easily fixed, so can the white dots. I'm gonna left it there.

Adalbert
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Re: Diatoms DIC

Post by Adalbert »

very nice :smt038 and good enough for me :D

anne
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Re: Diatoms DIC

Post by anne »

Absolutely wonderful!!
anne

PeteM
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Re: Diatoms DIC

Post by PeteM »

Skillfully done. Looks like your quest for DIC has been rewarded.

pbraub
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Re: Diatoms DIC

Post by pbraub »

Your result is very nice. Did you do any background subtraction or other correction?

micro_pix
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Re: Diatoms DIC

Post by micro_pix »

I don't think it gets much better than that. Looks like you've put together a very nice set-up!

Dave

Lou Jost
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Re: Diatoms DIC

Post by Lou Jost »

Beautiful and perfect!

Macro_Cosmos
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Location: Sydney

Re: Diatoms DIC

Post by Macro_Cosmos »

Thanks for the kind and motivating words! :D
pbraub wrote:
Thu Sep 24, 2020 12:53 pm
Your result is very nice. Did you do any background subtraction or other correction?
Yeah, for the second 100x stack. It was pretty easy since only one diatom was involved. It's decent without a background swap, all I had to do with stamp out the artefacts and/or use ZS' tools. You can see some of those artefacts in the 40x stack.

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