Diatom in brightfield & darkfield 42:1

Images made through a microscope. All subject types.

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Soki
Posts: 48
Joined: Sun May 03, 2020 5:05 am

Diatom in brightfield & darkfield 42:1

Post by Soki »

Hey guys,

i had my first darkfield experience days ago. I only have old random equipment and made the darkfield via a plastic-inlay made by an 3D-printer. Works pretty nice and is cheap.

I get more and more in love with photographing diatoms and hope you like it:)

Zeiss Jena microscope (1931)
Nikon D3400
Leitz Wetzlar 6FL C 42:1 0,85
(No eyepiece)
stack of 20, 8 & 40 single pictures
Attachments
CC4C1F15-9FFD-4920-90E8-9E1C9C08B315.jpeg
79E016C6-8E14-4CDB-8CE2-ED74B55C66F9.jpeg
E013B74E-B2F9-4D3E-A2A7-16F2A2A63F02.jpeg

grgh
Posts: 348
Joined: Sat Mar 09, 2013 4:55 am
Location: Lancashire. UK

Re: Diatom in brightfield & darkfield 42:1

Post by grgh »

Very nice shots, the blue in the second shot, how did you get that shade?
used to do astronomy.
and photography.
Zeiss Universal Phase contrast.
Zeiss PMII

Robert Berdan
Posts: 239
Joined: Wed Oct 18, 2017 8:58 pm
Location: Calgary
Contact:

Re: Diatom in brightfield & darkfield 42:1

Post by Robert Berdan »

Very nice darkfield images. Sometimes home made solutions are the best.
Cheers
RB

Smokedaddy
Posts: 1710
Joined: Sat Oct 07, 2006 10:16 am
Location: Phoenix, Arizona
Contact:

Re: Diatom in brightfield & darkfield 42:1

Post by Smokedaddy »

Well done! Very nice images.

Soki
Posts: 48
Joined: Sun May 03, 2020 5:05 am

Re: Diatom in brightfield & darkfield 42:1

Post by Soki »

Thank you 😊

grgh wrote:
Thu Jul 02, 2020 3:54 pm
Very nice shots, the blue in the second shot, how did you get that shade?
This was actually the „natural outcome“ with inside the camera white-balance. Stronger light changed the soft pink/purple tone in this bright violetish blue.

Here a comparison picture with the same settings despite an other lightning angle and less light.
Attachments
1D1088F6-F7B9-4930-9F7F-E10C9223D5BD.jpeg

grgh
Posts: 348
Joined: Sat Mar 09, 2013 4:55 am
Location: Lancashire. UK

Re: Diatom in brightfield & darkfield 42:1

Post by grgh »

Thank you for the info, I do like that blue shade though, at first i guessed at a halo effect but on closer inspection could see how wrong that was.

well done.
used to do astronomy.
and photography.
Zeiss Universal Phase contrast.
Zeiss PMII

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