Diatoms in Circular Oblique Light Update 06/05
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Diatoms in Circular Oblique Light Update 06/05
The 104 Forms type slide created by Klaus Kemp is still with me and I found the opportunity to experiment a bit more with darkfield, an 100x iris objective and (circular) oblique light. After a lot of fiddling around I found a setup that provided images with detail as well as a more 3-dimensional appearance. Here are the first six:
1 Arachnoidiscus ornatus
2 Auliscus sculptus
3 Triceratium favus
4 Amphitetras antediluviana
5 Eupodiscus radiatus
6 Coscinodiscus jonesianus
1 Arachnoidiscus ornatus
2 Auliscus sculptus
3 Triceratium favus
4 Amphitetras antediluviana
5 Eupodiscus radiatus
6 Coscinodiscus jonesianus
Last edited by WalterD on Tue May 05, 2020 10:27 pm, edited 5 times in total.
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- carlos.uruguay
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Thanks all for the kind remarks.
@Macro_Cosmos:
Yes I was glad to have the opportunity to buy this slide from Klaus Kemp, who's been a master in diatom preparation for decades.
And I don't know anybody else offering commercial type slides this good with this variety of types.
@ Lou:
The fiddling around included:
Changing over from the Ortholux II to the Orthoplan setup, abandoning the mirror house and speedlite flash setup, introducing a 10W understage led, switching over to 0,8Na darkfield condenser, determining the best condenser height, iris diaphragm opening and camera parameters.
Today I've added six more:
7 Artinoptychus splendens
8 Actinoptychus senarius
9 Stephanodiscus niagarae
10 Cocconeis pediculus upper valve (l) and lower.
11 Triceratium pentacrinus
12 Stephanodiscus hantschii
@Macro_Cosmos:
Yes I was glad to have the opportunity to buy this slide from Klaus Kemp, who's been a master in diatom preparation for decades.
And I don't know anybody else offering commercial type slides this good with this variety of types.
@ Lou:
The fiddling around included:
Changing over from the Ortholux II to the Orthoplan setup, abandoning the mirror house and speedlite flash setup, introducing a 10W understage led, switching over to 0,8Na darkfield condenser, determining the best condenser height, iris diaphragm opening and camera parameters.
Today I've added six more:
7 Artinoptychus splendens
8 Actinoptychus senarius
9 Stephanodiscus niagarae
10 Cocconeis pediculus upper valve (l) and lower.
11 Triceratium pentacrinus
12 Stephanodiscus hantschii
You're welcome, Lou!
The sensor of the camera is the CMOS type APS-C sized, which is "medium frame". The Periplan 6,3x 30mm eyepiece in this setup results in a large field number, FN28. The Objective is a 100x/ 1.32-0.6 iris immersion objective.
Below 2 pictures of the setup including the led light.
Before COL I tried differential interference contrast on the other scope (the Ortholux). Results were very colorful but also less realistic i.m.o.
The last image was also done with DIC but desaturated and inverted in Photoshop.
The sensor of the camera is the CMOS type APS-C sized, which is "medium frame". The Periplan 6,3x 30mm eyepiece in this setup results in a large field number, FN28. The Objective is a 100x/ 1.32-0.6 iris immersion objective.
Below 2 pictures of the setup including the led light.
Before COL I tried differential interference contrast on the other scope (the Ortholux). Results were very colorful but also less realistic i.m.o.
The last image was also done with DIC but desaturated and inverted in Photoshop.
- iconoclastica
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Did you put a stop on the field lens? Totally amazing ... jaw dropping to say the least. Very 3D like, not to mention the resolution. Sure wish I could come close to any of those images.
-JW:
-JW:
Last edited by Smokedaddy on Sat May 02, 2020 12:35 am, edited 1 time in total.