classic microscopy, stained cut of Casuarina

Images made through a microscope. All subject types.

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anne
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classic microscopy, stained cut of Casuarina

Post by anne »

Stained cuts of Casuarina:

Image

Image

Image

and in Polarization:

Image

BR
Anne

75RR
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Post by 75RR »

Beautiful images. Can you give some details on the microtome.
Zeiss Standard WL & Wild M8
Olympus E-p2 (Micro Four Thirds Camera)

Smokedaddy
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Post by Smokedaddy »

Excellent

Jacek
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Post by Jacek »

Very nice

ChrisR
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Post by ChrisR »

Impressive staining work, Anne.:)
Can you share your recipes?
Chris R

anne
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Post by anne »

Hello,
I have to tell you I am only the photographer. This very rare cuts are from the famous Robin Wacker. A friend was so kind to stain them for me and to mount them.
The staining is done with W3A sym 1, a special formulation developed by Robin Wacker and for a long time a secret of him.
You can find it on page 210 here:

https://www.zobodat.at/pdf/Mikrokosmos_95_4_0001.pdf

BR
anne

grgh
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Post by grgh »

Anne
wonderful images.

Any chance you could get an English translation of the PDF posted,
looking through the pages and all look interesting, when i try Google translate, I receive notice that the file is to long.
used to do astronomy.
and photography.
Zeiss Universal Phase contrast.
Zeiss PMII

WalterD
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Post by WalterD »

Beautiful images Anne. The staining is excellent.
I came across the name Wacker before and... by coincidence am attempting to use a rotary microtome for botanical sections.

Regards,

Walter

RogelioMoreno
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Post by RogelioMoreno »

Anne,

Beautiful set! My favorite is #2, what objective?

Rogelio

anne
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Post by anne »

Walter: Thank you!
Rogelio: I used the 20 SPlanApo Olympus. It is a Stitch of 9 Panels. Thank you!
BR
Anne

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