Hi,
i am toying around with my new UV excitation autofluorescence setup and took some garden variety images.
Twig of a privet hedge (UPlanFL4x).
Ghostberry (UPlanFL4x).
The last image is a berberis berry with an incidental fungal infection taken with a UPLanFLN10x objective. The fungus was not visible with the naked eye and only revealed itself by its strong blue-ish autofluorescence.
The images were taken with PlanFL 4x and 10x lenses on an Olympus BX61 controlled with Micro-Manager 2 gamma. Illumination came from a UV LED (CoolLED pE300) with a Semrock DAPI EX/BS and a 408 nm LP EM filter. The camera was a Point Grey color camera.
Images were stacked in Zerene (i used the holiday deal, thank you!).
My next plans are to establish size calibration and optimize the stacking protocols.
I hope you like my images and I am looking forward to constructive criticism am discussion.
Kind regards
Peter
Garden variety autofluorescence
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- carlos.uruguay
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