anomoeoneis sphaerophora

Smokedaddy · Post by **Smokedaddy** » Wed Oct 11, 2017 8:29 am

Phase contrast.

Transmitted DIC.

-JW:

Pau · Post by **Pau** » Wed Oct 11, 2017 9:12 am

Very nice DIC image, but I don't like the yellow-brown tone, do you see it through the eyepieces? In any case it can be corrected with custom WB at the camera or at RAW conversion...if you neither like it.

Smokedaddy · Post by **Smokedaddy** » Wed Oct 11, 2017 10:43 am

Thanks, I liked the DIC image better as well. This was my first attempt using Phase with my newly acquired Nikon DIC Achr/Apl 1.35 DIC condenser. Those are the true colors on both images but I did 'smooth' out the background considerably with the stamp tool and to get rid of all the dust motes. There was no hue or saturation used. The phase reminded my of anoptral phase setup used on my PZO Bioilar.

carlos.uruguay · Post by **carlos.uruguay** » Wed Oct 11, 2017 10:50 am

Wooowwww!!!! Super!!!

Smokedaddy · Post by **Smokedaddy** » Wed Oct 11, 2017 10:53 am

Thanks Carlos.

hkv · Post by **hkv** » Wed Oct 11, 2017 11:17 am

The second one has great details! Nice work!

Jacek · Post by **Jacek** » Thu Oct 12, 2017 12:07 pm

Very nice

WalterD · Post by **WalterD** » Sun Oct 15, 2017 9:31 am

Lovely, thanks for sharing!

Smokedaddy · Post by **Smokedaddy** » Sat Oct 12, 2019 7:11 pm

... I forgot to post this one. Just messing around. Obviously the black is done in Photoshop. Larger image is here;

https://squattingdog.smugmug.com/Diatoms/i-TK3w9Jk/A

-JW:

MASZEK · Post by **MASZEK** » Sun Oct 13, 2019 1:35 am

Your photos are a good comparison of two observation techniques. I wondered for a long time which shot is nicer in my opinion. Finally, I chose DIC. This technique gives more elegant effects.
Some time ago I scraped off some stone residue and observed many diatoms in a bright field. But it was a failure. DIC or Ph are much better

Smokedaddy · Post by **Smokedaddy** » Wed Oct 16, 2019 6:36 pm

In most cases I totally agree.

Ichthyophthirius · Post by **Ichthyophthirius** » Thu Oct 17, 2019 6:36 am

Hi,

The DIC image is very nice. Did you use the DIC-A slider or do you have a special objective-side prism for the Planapo 40/0.95?

The Nikon phase contrast is very colourful

But it wouldn't be fair to compare it directly to the DIC as it's an achromat vs. a plan apo with almost twice the NA.

Smokedaddy · Post by **Smokedaddy** » Thu Oct 17, 2019 8:25 am

Ichthyophthirius wrote:Hi,

The DIC image is very nice. Did you use the DIC-A slider or do you have a special objective-side prism for the Planapo 40/0.95?

The DIC image was with my Optiphot and this setup.

-JW:

grgh · Post by **grgh** » Thu Oct 17, 2019 3:10 pm

Both nice shots,but,
first shot electrifying, reminded me of old valves from radio days.

Smokedaddy · Post by **Smokedaddy** » Thu Oct 17, 2019 3:25 pm

grgh wrote:Both nice shots,but,
first shot electrifying, reminded me of old valves from radio days.

I can relate to that.

-JW: