anomoeoneis sphaerophora

Images made through a microscope. All subject types.

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Smokedaddy
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anomoeoneis sphaerophora

Post by Smokedaddy »

Phase contrast.

Image

Transmitted DIC.

Image

-JW:

Pau
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Post by Pau »

Very nice DIC image, but I don't like the yellow-brown tone, do you see it through the eyepieces? In any case it can be corrected with custom WB at the camera or at RAW conversion...if you neither like it.
Pau

Smokedaddy
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Post by Smokedaddy »

Thanks, I liked the DIC image better as well. This was my first attempt using Phase with my newly acquired Nikon DIC Achr/Apl 1.35 DIC condenser. Those are the true colors on both images but I did 'smooth' out the background considerably with the stamp tool and to get rid of all the dust motes. There was no hue or saturation used. The phase reminded my of anoptral phase setup used on my PZO Bioilar.

carlos.uruguay
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Post by carlos.uruguay »

Wooowwww!!!! Super!!!

Smokedaddy
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Post by Smokedaddy »

Thanks Carlos.

hkv
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Post by hkv »

The second one has great details! Nice work!

Jacek
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Post by Jacek »

Very nice

WalterD
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Post by WalterD »

Lovely, thanks for sharing!

Smokedaddy
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Post by Smokedaddy »

... I forgot to post this one. Just messing around. Obviously the black is done in Photoshop. Larger image is here;

https://squattingdog.smugmug.com/Diatoms/i-TK3w9Jk/A

Image

-JW:

MASZEK
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Post by MASZEK »

Your photos are a good comparison of two observation techniques. I wondered for a long time which shot is nicer in my opinion. Finally, I chose DIC. This technique gives more elegant effects.
Some time ago I scraped off some stone residue and observed many diatoms in a bright field. But it was a failure. DIC or Ph are much better

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Post by Smokedaddy »

In most cases I totally agree.

Ichthyophthirius
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Post by Ichthyophthirius »

Hi,

The DIC image is very nice. Did you use the DIC-A slider or do you have a special objective-side prism for the Planapo 40/0.95?

The Nikon phase contrast is very colourful :) But it wouldn't be fair to compare it directly to the DIC as it's an achromat vs. a plan apo with almost twice the NA.

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Post by Smokedaddy »

Ichthyophthirius wrote:Hi,

The DIC image is very nice. Did you use the DIC-A slider or do you have a special objective-side prism for the Planapo 40/0.95?
The DIC image was with my Optiphot and this setup.

Image

-JW:

grgh
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Post by grgh »

Both nice shots,but,
first shot electrifying, reminded me of old valves from radio days.
used to do astronomy.
and photography.
Zeiss Universal Phase contrast.
Zeiss PMII

Smokedaddy
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Post by Smokedaddy »

grgh wrote:Both nice shots,but,
first shot electrifying, reminded me of old valves from radio days.
I can relate to that. 8)

Image

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