SEM: Dandelion pollen

Images made through a microscope. All subject types.

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flyer2o12
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SEM: Dandelion pollen

Post by flyer2o12 »

Hey guys,

How I would love to win the lottery buy my own SEM!! An absolute dream come through it would be. Here's a couple of scans I did a while back of Taraxacum pollen.

Suggestions for subjects and feedback welcome.

Image

Image

Image

Image

Apologies for the poor image quality. The site does not allow me to upload greater resolution. They can however be seen on my Flickr page.

Thanks!

Jacek
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Post by Jacek »

wonderful :D

siliconGary
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Post by siliconGary »

I have toyed with the idea of owning a SEM over the years - never bit but every so often think about it. I have seen several 80's models in the $5-10k range (But shipping is going to really cost.) Here is one that sold for US$1400 https://www.govdeals.com/index.cfm?fa=m ... cctid=7218 that is less than quite a few optical set-ups.
I bought and used a Cambridge Instruments Stereoscan at work back in the eighties. You would probably need to spend a few hundred over-hauling the pumps but would very likely still get a decent image off of it up to ~30-50,000x. The Stereoscans where good in that you didn't need complicated hook-ups with nitrogen/compressed air etc. - you just plugged them in to the socket. Standard filaments so vacuum requirements are not too bad (compared to field emission) and they had a small neat turbo pump. But of course you still need to buy a gold coater, and they are at least another $1000 (And a new target will double that)

Choronzon
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Post by Choronzon »

The problem as I understand it is digital capture on these older models.
There isn't any. And the software firmware would cost more than the sem.
I am not young enough to know everything.

Sumguy01
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Post by Sumguy01 »

:smt038 amazing set.
Thanks for sharing.

René
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Post by René »

Lovely images, Karl!
siliconGary wrote:Here is one that sold for US$1400 https://www.govdeals.com/index.cfm?fa=m ... cctid=7218
Hi Gary, around 2002 I have started up exactly this machine in a botanical lab, after storage for a few years. I remember having to call the service tech quite a few times due to failing chip sets. Unless you're into electronics as well, I wouldn't advice on such an adventure ;-)

I suppose the first edition of tabletop SEMs will come up on the secondhand market soon. Up to 10.000x (only), but at least the electronics are still fresh.

Best wishes, René

Lou Jost
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Post by Lou Jost »

Wow, how did you get the colors?

carlos.uruguay
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Post by carlos.uruguay »

SUPER!!!!

pwnell
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Post by pwnell »

Well done!

JH
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Post by JH »

Nice pictures and interesting!
I like the colorizing - well done. Is this a totally manual process in Adobe PS or some similar program?
Regards Jörgen
Jörgen Hellberg, my webbsite www.hellberg.photo

flyer2o12
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Post by flyer2o12 »

JH wrote:Nice pictures and interesting!
I like the colorizing - well done. Is this a totally manual process in Adobe PS or some similar program?
Regards Jörgen
Hi,

Yes it's an entirely manual process in PS. Glad you like them.

houstontx
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Post by houstontx »

how do you sputter coat? Do you use gold? Have you sputter coated with carbon powder?

Thanks,

flyer2o12
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Post by flyer2o12 »

For these SE images I sputter coat just with gold.

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