Bright field Daphnia

CEN · Post by **CEN** » Tue Sep 20, 2016 6:51 am

Good afternoon (GMT +2h),

here I present two images of Daphnia with (simple) bright field ilumination. Both shots were made with an Olympus BHT stand equipped with a SPlan 4 and a NFK 2.5.

Lateral view:

Ventral view:

Regards

Christian

JH · Post by JH » Tue Sep 20, 2016 8:16 am

Nice, clean and in perfect orientation. How did you preparera the slide?
Regards Jörgen

hkv · Post by **hkv** » Tue Sep 20, 2016 8:53 am

Great shot! Ditto Jörgens question!

carlos.uruguay · Post by **carlos.uruguay** » Tue Sep 20, 2016 10:13 am

Nice!

CEN · Post by **CEN** » Tue Sep 20, 2016 11:03 am

Hi there

Many thanks for your comments.

The preparation of the slide was very easy. First I killed the Daphnia in 70 % Ethanol, then I transferred it into a drop of water on a slide. I used a cover glass with beeswax spacers, thus I had a thick layer of water. By slightly moving the cover glass I was able to "rotate" the Daphnia into an appropriate position. A well known technique, I guess?

Regards

Christian

JH · Post by JH » Tue Sep 20, 2016 12:19 pm

Hi Christian

Thanks for the info!

CEN wrote: I used a cover glass with beeswax spacers, .... A well known technique, I guess?

I have not, but I would like to know more. Is this a ring/square of melted beeswax applied to the glass or wax beads or something else?

Regards Jörgen

CEN · Post by **CEN** » Tue Sep 20, 2016 1:09 pm

Hi Jörgen

It is a really primitive technique. First you have knead some beeswax until it is warm enough to form it to small balls. Then you have to press these wax balls on the cover glass until they have the required height. I have prepared various cover glasses for different distances.

Here just a scetch, prepared within a minute with PS:

The yellow bubbles are the beeswax spacer. This technique is described in any German Book for Amateur-Microscopy.

I hope this was helpful for you

Kind Regards

Christian

JH · Post by JH » Tue Sep 20, 2016 1:22 pm

Hi Christian

Thanks again for the info!

Regards Jörgen

Sumguy01 · Post by **Sumguy01** » Tue Sep 20, 2016 8:49 pm

Very nice.
Thanks for sharing.

Jacek · Post by **Jacek** » Wed Sep 21, 2016 1:14 am

Very nice

CEN · Post by **CEN** » Wed Sep 21, 2016 4:24 am

Thanks, Jacek and Sumguy01

Charles Krebs · Post by **Charles Krebs** » Thu Sep 22, 2016 12:30 am

Excellent! Crisp, clean, detailed!

piermicro · Post by **piermicro** » Thu Sep 22, 2016 11:28 am

Very nice.

CEN · Post by **CEN** » Thu Sep 22, 2016 1:04 pm

Thanks, Charles and grazie mille, Pierangelo!

RogelioMoreno · Post by **RogelioMoreno** » Fri Sep 23, 2016 1:43 pm

Very nice!

Rogelio