This is the first test using this objective which is only temporarily set up for brightfield epi. Image is of a jewel beetle wing case where the two come together on the back. You can just see the edge at the top.
Nikon CFI Plan 20x 0.4 BD ELWD, Nikon CF PL 2.5x, ZS Dmap. The stack could have been a bit deeper, something to correct next time. Comments welcome.
Edit: Add darkfield stack of the same part. The wing case appears to be covered in tiny transparent blobs like lenses which may account for it's irridescence. But after reading this highly technical paper - probably not.
http://rstb.royalsocietypublishing.org/ ... 6/1565/709
Beetle wing case at 50x
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Re: Beetle wing case at 50x
The colors are structural colors, induced by the light difraction at much smaller scale inside the cuticulesteveminchington wrote:The wing case appears to be covered in tiny transparent blobs like lenses which may account for it's irridescence. But after reading this highly technical paper - probably not.
Actually the bolbs are droplets, maybe of water or if the specimen is dry of oil. You can clean it with alcohol or with a strong detergent solution.
The droplets acting as lenses induce some stacking artifacts more visible at the central part of the BF image
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