Closterium cell wall

Images made through a microscope. All subject types.

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NikonUser
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Closterium cell wall

Post by NikonUser »

I was about to photograph a live Closterium (a desmid) when I applied a little too much pressure on the coverslip. The cell wall ruptured and all the contents flowed out leaving a transparent cell; actually 2 hemicells joined centrally.
These images show the brownish cell walls lined with longitudinal striations and some girdle bands.
Oly SPlan 20x and 40x, 3.3x NFK relay lens, full frame Nikon D600, Zerene Stacker PMax. Water mount.
Forgot to mention: DIC with 1.25x auxillary lens.
Image
Image
NU-13-03-05
NU.
student of entomology
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” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
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René
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Post by René »

Wow, never saw it as clear as that!

Thanks, René

NikonUser
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Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Post by NikonUser »

Thanks, René: I have looked at a lot of live Closterium and also had never seen the cell wall in such detail. I think my clumsy mounting turned out to be an advantage.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

naturephoto1
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Post by naturephoto1 »

Superb.

Rich

discomorphella
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Post by discomorphella »

That's great. I am going to have to attempt to "put the squeeze" on some desmids now.

David

NikonUser
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Location: southern New Brunswick, Canada

Post by NikonUser »

Thanks Rich & David.
I don't recall ever seeing such marked longitudinal striations and girdle bands in live specimens; may have been very lucky with this species.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

flyer2o12
Posts: 220
Joined: Mon Jan 09, 2012 5:06 pm
Location: Ireland
Contact:

Post by flyer2o12 »

Very, very nice images!! The detail is incredible!

There is one minor mistake in your images though, you have ""longitudinal straitions are 80 micro apart" That should be 80 micro meters. :wink:

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