Fluorescent photography - water fleas, rotifers, hydras

Images made through a microscope. All subject types.

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Starshade
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Joined: Wed May 04, 2011 1:47 am

Fluorescent photography - water fleas, rotifers, hydras

Post by Starshade »

Hello everyone, I am new to photomicrography and the last 4 month I've been doing fluorescent photography. Just few examples of it:

Image
That's a hydra

Image
And that's a daphnia

As the forum has limits on uploading stuff, it's easier for me to give a link to my gallery http://www.behance.net/gallery/Fluoresc ... ds/1790456

Most of pictures are stacks with modified background
Last edited by Starshade on Fri Jul 22, 2011 12:25 am, edited 3 times in total.

arturoag75
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Post by arturoag75 »

Well done!
very interesting shoots and technique!
best
arturo

Starshade
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Joined: Wed May 04, 2011 1:47 am

Post by Starshade »

Thank you,

Basically the technique is epifluorescence, but taking pictures was very difficult. One of the main challenges was stacking as stacking software is unable to recognize what's going on in the photos, so I did everything manually.

peter-h
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Post by peter-h »

Hello,
great images ! Please can you tell us some more about your technics. Have you used a UV-LED for this fluorescence and what wavelengh have you used? Have you used Acridinorange?

Regards
Peter

Simon W
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Post by Simon W »

really nice images in your site! Talk about exotic colours and details
Simon W
EOS 5D Mk 3; Olympus BH-2; Zerene Stacker
Melbourne, Australia

Starshade
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Joined: Wed May 04, 2011 1:47 am

Post by Starshade »

Thank you,

I think I'll be posting more and telling about techniques.

The UV lamp I used is the cheapest mercury one, wish I had something better.

Then the key part is using fluorescent dyes - sometimes more than one. Acridine orange is seldom useful at least alone, only oligochaeta and two rotifers are coloured with AO alone.

Harald
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Post by Harald »

Hi there starshade,
Just love your pictures. Fluorescent photography through the microscope is facinating. Your images is of very good quality. Thanks for sharing :D
Kind Regards
Harald

Lier Fotoklubb / NSFF
AFIAP / CPS
BGF / GMV
http://www.500px.com/blender11

Ecki
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Post by Ecki »

Great pictures! Which other fluorochromes are you using besides Acridine Orange?

Starshade
Posts: 133
Joined: Wed May 04, 2011 1:47 am

Post by Starshade »

I tried many options, propidium iodide can give good light for example. I will not list every single dye I tried, basically I ended up having very dark images and super long exposures don't give acceptable quality.

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