Dismids and diatoms

Images made through a microscope. All subject types.

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Linden.g
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Dismids and diatoms

Post by Linden.g »

A few more from a marsh and pond. 100x Olympus S-Plan Achromat, flash DIC.
Image

Marek Mis
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Post by Marek Mis »

Hello Linden,

Very nice image.
Do you use only plan achromats, not plan apochromats ? The details on your images are very fine. I didn't expected that the plan achromats work so good. Perchaps it depends on the rest of optics in other parts of microscope - for example in the trinocular head. I use CZJ older microscope and I'm not so satisfied of my results if about Olympus plan achromats (I use 20 X and 40 X ones).

Regards

Marek Mis
Suwalki

http://www.mismicrophoto.com/

Mitch640
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Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

The details are amazing, especially that first diatom at the top. I was also wondering, what kind of image would you get if you did the same shot in Brightfield. Is it the DIC that provides the detail?

NikonUser
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Location: southern New Brunswick, Canada

Post by NikonUser »

Mitch:
There are a few examples of somewhat similar non-DIC images on these 2 pages:
HERE
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Mitch640
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Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Maybe it's the colors. Both seem to have good detail, but just in different ways. It would be interesting to see the same subject under the different lighting though.

twebster
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Post by twebster »

I love your composite images. The background is so even shaded that the image looks like a single image, not like an image made of several different images put together. Well done on the technique. :D
Tom Webster

Phoenix "The Valley of the Sun", Arizona, USA

The worst day photographing dragonflies is better than the best day working! :)

NikonUser
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Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Post by NikonUser »

Mitch640 wrote:Maybe it's the colors. Both seem to have good detail, but just in different ways. It would be interesting to see the same subject under the different lighting though.
OK, I don't see much difference when I use DIC for green algae as compared with regular brightfield. But then I have a pretty lousy DIC system. DIC seems particularly useful for showing up structures in an otherwise clear protoplasm such as protozoa and onion cells.
See below for a direct comparison of same subject:
DESMID HERE.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Ah, OK, it seems to highlight small differences with a shadow/oblique effect to bring out the detail. It's not surprising I am having a hard time wrapping my head around this, it took me weeks to figure out Arcella did not have a broken shell, it was just the jelly inside. LOL

Linden.g
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Post by Linden.g »

Thanks Tom, I like the composites they remind me of text book graphics.

Marek, I have a full set if achromats but no Apos. I also have a matched set of DIC optics, take a look here http://www.flickr.com/photos/13084997@N ... 8697012197. All the above images were photostacks processed using Zerene Stacker with prior removal of chromatic aberration during RAW conversion.

Mitch, I think you see more surface relief with DIC but the advantage is much less on dense coloured organisms.
Linden

Marek Mis
Posts: 2584
Joined: Sat Jul 10, 2010 9:56 am
Location: Suwalki, Poland
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Post by Marek Mis »

Linden,

Thank you very much for your info. I've just looked over your microphotographs - they are superb ! Congratulations.
Regards

Marek
Suwalki

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