An aster floret cross sections

Images made through a microscope. All subject types.

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blepharopsis
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An aster floret cross sections

Post by blepharopsis »

A cross section through a disc floret, one of 2 dozens or so within a flower bud of some relative of a daisy I found on the campus lawn. Embedded in agarose, sectioned at @200 um, stained with Congo Red and Calcofluor White.

aster-1c-cleared-25x1-gigapixel-standard-scale-2_00x-2.jpg
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aster-1a-25x-1-gigapixel-low_res-scale-2_00x.jpg
aster-1e-40x-cleared-1-boosted1.jpg
aster-1e-40x-cleared-1-boosted-2.jpg
aster-1e-40x-cleared-fire-gigapixel-low_res-scale-2_00x.jpg-1.jpg
aster-1e-40x-cleared-fire-gigapixel-low_res-scale-2_00x-2.jpg
aster-new-40x-1-gigapixel-standard-scale-2_00x2a.jpg
aster-new-40x-1-gigapixel-standard-scale-2_00x2b.jpg

Adalbert
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Re: An aster floret cross sections

Post by Adalbert »

very nice :smt041


Harald
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Re: An aster floret cross sections

Post by Harald »

Amazing!
Kind Regards
Harald

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Macro_Cosmos
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Re: An aster floret cross sections

Post by Macro_Cosmos »

Simply amazing, this is a candidate for Nikon's SmallWorld contest, I think you'll get at least an honourable mention.
This is just beautiful. Did you use a rotary microtome? I would love to learn your staining protocol too.

blepharopsis
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Re: An aster floret cross sections

Post by blepharopsis »

Thank you :)
I'm using Leica Vibratome, it is very versatile, as long as you embed in ~7% agarose and your sections are pretty thick (>50 um). My staining protocol is simple: Calcofluor White (0.1 mg/ml, Sigma-Aldrich #F3543-1G), Congo Red (0.5 mg/mL, Sigma-Aldrich #C676-25G) (I eyeball the amounts at this point :)) in PBS-T (PBS + 0.1-0.5% Triton X-100, or any non-ionic detergent). Overnight, RT. Works for cellulose-containing samples as well as for chitin (invertebrate exoskeletons). CR has more apparent affinity for more tightly packed/sclerotized cellulose/chitin. CW stains preferentially (again, my own observation) thinner/more flexible cellulose/chitin. Hope that helps!
Macro_Cosmos wrote:
Mon Oct 03, 2022 3:24 pm
Simply amazing, this is a candidate for Nikon's SmallWorld contest, I think you'll get at least an honourable mention.
This is just beautiful. Did you use a rotary microtome? I would love to learn your staining protocol too.

jmc
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Re: An aster floret cross sections

Post by jmc »

Absolutely amazing photos. Thanks for sharing.
Jonathan Crowther

AlP
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Re: An aster floret cross sections

Post by AlP »

Beauty, most excellent.

houstontx
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Re: An aster floret cross sections

Post by houstontx »

Probably the best cross section image ive ever seen, amazing, thank you for posting!

Pau
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Re: An aster floret cross sections

Post by Pau »

:shock: =D> =D> =D>
Pau

Sumguy01
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Re: An aster floret cross sections

Post by Sumguy01 »

=D> Very nice.
Thanks for sharing.

Vincent Zhang-1968
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Re: An aster floret cross sections

Post by Vincent Zhang-1968 »

it's awesome ! :D

discomorphella
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Re: An aster floret cross sections

Post by discomorphella »

Igor these are just beautiful. Thanks for posting.
Best
Dave

Ramos Kenneth D
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Re: An aster floret cross sections

Post by Ramos Kenneth D »

An excellent example of the hidden intricacies of nature 8)

Smokedaddy
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Re: An aster floret cross sections

Post by Smokedaddy »

Incredible, to say the least.

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