I haven't been to successful with the PZO setup, even in BF and not at all in DIC. Before I even ask about DIC I have the following questions. To be clear, I am under the impression that; 1. The condenser centering screws I am pointing at in the 3rd image below center the field illumination diaphrag...

... different objective, GIF filter, figured I'd leave it green and make the border green too. <g>



-JW:

Have you tried the technique of using diffused darkfield illumination, sent in from the sides instead of up through a condenser? --Rik Thanks ... I actually saw all of those original posting but didn't read the newer postings of the technique used. I was even discussing that with Curt a couple week...

Thanks ... I suppose where I'm frustrated is the image looks more like a charcoal pencil sketch from my perspective.

Thanks, I've never used that option before. I'll have to give it a try sometime.



-JW:

Been struggling with this for a couple of days now. Can't seem to get what I want. Any actual imaging tricks are more than welcome.



* Image updated (wrong objective was listed)

Incredible colors, amazing creatures. What was the specific objective 10x etc.?

...very interesting images.

Thanks for the .PDF's John, they will be extremely helpful. Pau I agree with you 100%. Thanks for the Ziess booklet link too which I definitely need. Lastly a big thanks to JohnyM outlining the steps and calling out the components in my photo. All of your information and suggestions are very much ap...

Well I got this far today. I am not sure what the next step is? I didn't want to try any oil objectives until I understand the settings. If any PZO folks 'in the know' have suggestions for 'setting' for the numbered items to get me in the ball park I would appreciate it. I should mention that I've m...

Getting closer to at least testing the setup. The LED illuminator from Wlodek should be here early next week. The next step will be figuring out the DIC arrangement and how to use it. Looking forward to this. http://www.photomacrography.net/forum/userpix/153_DSC_2755_1.jpg http://www.photomacrograph...

Second attempt. Image zoomed 100% in PS then cropped, Nikon PlanApo CFN 60x/1.40, oiled slide and condenser, condenser closed to smallest NA, GIF used, then manipulated in PS. The diatom is 55 um in diameter, and the dots were about 0.459 um in diameter. http://www.photomacrography.net/forum/userpix...

I tried to photograph this several times but could never get the sharpness I wanted. This particular try (which was the best) was BF with 'no' GIF. I opened the condenser (from a fully closed position) until what I 'thought' looked the best. Which was very little. Both sides of the slide were oiled ...