Absolutely beautiful autofluorescence images.

David

Spectacular photos. At my altitude in the Oregon coast range Digitalis is up and buds have appeared but no flowers yet. I will have a new appreciation of them now.

David

That's a great haul for a plankton net. Beautiful.

David

Beautiful. I especially like the DF view of the ostracod.

David

Sanctified digestive end products! That's just spectacular.

David

A most spectacular combination of polarized optics, botany and computer code....Great shot.

David

the simplest way to correct in imageJ if you have a high SNR image and a good blank image is to use the image calculator plus and divide the original by the background. This will fail miserably if you have a noisy image (I would guess that SNR > 20 at least would be required but I'd have to sit down...

Phosphate buffered 4% formalin is a good routine fixative that is also a decent medium for storage of histological and botanical samples. There are several equivalent recipes but the simplest one is to dilute reagent 37% HCHO with pH 7.4 0.1M NaH2PO4 / 0.1M Na2HPO4 (standard Soreson's 0.1M phosphate...

Hi, I've used both commercial and some semi-homebrew 3D MLE (aka blind) deconvolution. I've tried both the AutoQuant (now Media Cybernetics) and Huygens commercial s/w both with good results, and I have dabbled with some of the code available for both MATLAB and imageJ in the public domain. I'm most...

Very nice. What does the cotton look like with a quarter-wave plate in the illumination optical path?

David

Beautiful images. You can also get tartaric and citric acid in reasonably pure form from cheese making places too. Inexpensive enough to recrystallize from DI water if you really want to.


David

Although it's tempting to think that the larger aperture condenser will have fewer aberrations for the marginal rays, this is highly dependent on the optical design. For many simpler lens systems where you can have significant spherical aberration, the rays that travel from the object to the outer e...

Spectacular. Where did you collect the specimen?

David

Awesome combination of history of science and microscopy. Its amazing what Leewenhoek was able to discern squinting through his comparatively tiny lens. I wonder what he'd say if he could glance through a pair of 28 mm FOV oculars...
Great article.

David

Hi js, Yes, I have been pretty lazy about post processing. I shoot in raw mode and then just usually very simply correct using NXD and then imageJ. The mosaics were composed of tens of images and I was in a hurry, so I didn't do much but copy/paste and add a sigma ~=20 pixel Gaussian blur to clean u...