These are remarkable. Did you use 365nm light? Thanks Lou Nope, just an Amaran 60d - a compact 65W daylight COB studio lamp - shining directly into the back of my old dome/ping-pong ball diffuser. And a little bit of frequency separation sharpening in post Diffuser is described here https://www.pho...

Thanks both, kinda. Demanding beggars, ain'tcha!? :D So now I have to admit that the forms were more widely separated in reality and I moved them closer together in post. :oops: <blush> I also note "tilted back 35-40 degrees", so of course I would love to see stereo. <insert begging puppy emoticon h...

A pleasing outcome from a test shot (after significant modding of my macro setup - another story). Shot with a 20x Mitutoyo M Plan APO on a Raynox DCR250 for 12.5x on sensor. I was really surprised to get such clear detail from this, albeit with very heavy cropping. The diatoms were stuck face up on...

... Their high precision 1.5 ones which has the right thickness, does not seem to come in small sizes, 10 mm is the smallest https://www.marienfeld-superior.com/precision-cover-glasses-thickness-no-1-5h-tol-5-m.html Can you trim them yourself without shattering them I wonder? Yes, a light scoring w...

That is, diatoms still buried in a tiny crumb of diatomite from Newport Bay, CA SMB00017--pmn.jpg Here's a close up focus stack from the centre of the crumb. Magnification 12.5x on sensor (20x Mitty on 125mm Raynox DCR 250). FoV covers about 1.6mm by 1.4mm. diatomite closeup - pmn.jpg Full resolutio...

Tragic news. I only ever interacted with Robert on this forum, but will still remember him as a friend. Always so affable and generous with his knowledge. He will be sorely missed. Beats.

Spectacular, as always. These images are very useful in helping "interpret" what I see optically too. The inside/outside differences in Didymosphenia (aka Rock Snot) are near impossible to separate with transmitted illumination, even using UV (you see both overlaid). But after seeing the SEM version...

...I have several brands of clean cotton pads and such that I sometimes use instead of Qtips, but I still check for residues since I found some that claim they leave no residues or lint or anything but they really did. Agreed! "Lint and residue free" too often means you simply weren't charged for t...

Salt helps to break down grease. A key constituent of many old (traditional) grease removal methods. It has caused a few to hyperventilate on seeing it in the recipe though :D But if applied as per the instructions - dampen (don't wet) a cloth and clean with that, then polish with a separate dry clo...

... another stuff what works excellent on fatty fingerprints is ROR Residual Oil Remover . It is water based and does not harm plastics like acetone. (Be very careful with acetone, it can instantly ruin plastic because it is vary strong solvent and you don't want to find your acetone wetted cleanin...

Great details from an interesting (and pretty uncommon) species. That's some serious "flexing" with the 2µm scale bars too - a mere four-and-a-bit (blue) wavelengths long!

I'm content with what my Sony bodies can do in studio macro and micro contexts, but I would like true 16-bit RGB outputs (instead of the current 14-bit max, which can drop to 10-bit in certain modes on older bodies). I shoot a lot of low-contrast micro subjects (mainly diatoms) and then stretch the ...

Always nice to see diatoms in their natural state. Very nicely captured. Cheers

Many species of diatoms live in the ocean. Some fish eat (or ingest) diatoms. Seabirds eat fish - then poop. The poop piles up in thick guano deposits. Some humans dig this guano up to use as fertiliser Other humans prefer to extract the diatoms and photograph them. Like this (100x/1.4 objective, 36...

hkv wrote: ↑

Sat Feb 24, 2024 3:25 pm

Smokedaddy wrote: ↑

Fri Feb 23, 2024 10:26 am

Excellent and a superb job coloring.

Thank you! Coloring is the most time consuming part of SEM imaging.

Similarly, achieving such depth of field is the most time consuming part of optical imaging

Great image with loads of fascinating detail. Keep 'em coming please!

Cheers
Beats