Search found 82 matches

by pbraub
Tue Jan 22, 2019 11:37 am
Forum: Equipment Discussions
Topic: Fluorescence on Optiphot
Replies: 34
Views: 11613

The HBO Lamps need some time to stabilize after ignition (15-20 min), this is especially relevant if you want to take stacks. Do not turn off immediately after ignition and do not re-ignite a hot lamp. Both shortens the lifespan. The olympus BX-RFA manual also says #To extend the mercury burner life...
by pbraub
Wed Jan 16, 2019 9:25 am
Forum: Equipment Discussions
Topic: Fluorescence on Optiphot
Replies: 34
Views: 11613

As Pau said, thank you very much Johny.

Do you have optics beside a mirror in your 3-way Cube? I am thinking about building a similar thing to mount an HBO and a LED at the same time. It would be nice to switch them without much mechanical work. Any would be appreciated.

Kind regards
Peter
by pbraub
Wed Jan 16, 2019 5:11 am
Forum: Equipment Discussions
Topic: Fluorescence on Optiphot
Replies: 34
Views: 11613

Hi, there is a paper out detailing a LED illumination axis made with simple off the shelf components (Arduino, Thorlabs, etc.). They also have a comparision of critical and Koehler illumination: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0143547 Edit: I think besides having le...
by pbraub
Mon Jan 14, 2019 1:52 am
Forum: Equipment Discussions
Topic: "Special objective" for centering epi illuminators
Replies: 6
Views: 2980

Hi, I also found the centering procedure outlined in the olympus RFA manual produced sufficiently good results for me. Olympus advises the use of a centering target (U-CST) which I don't have and always replaced by a sheet of cardboard with a cross drawn on it or just by a plain sheet of paper/cardb...
by pbraub
Fri Jan 11, 2019 1:14 am
Forum: Photography Through the Microscope
Topic: Various
Replies: 22
Views: 6202

Jacek! Thank you for the info.
What Diodes do you use? Are you willing to share the information.

Your new pictures are stunning (and it is somewhat comforting to see that I am not the only one having problems with stray highly fluorescent fibres).

Peter
by pbraub
Thu Jan 10, 2019 12:40 pm
Forum: Photography Through the Microscope
Topic: Various
Replies: 22
Views: 6202

Spectacular images, thank you Jacek!

What lightsource do you use for UV excitation? Do you excite through the objective or from the side?

Kind regards
Peter
by pbraub
Mon Dec 31, 2018 4:44 am
Forum: Photography Through the Microscope
Topic: Diatoms from Barbados
Replies: 13
Views: 2855

Anne - why the question marks?

I think your microphotographs have a very distinct style - both in the technical execution and in the framing of the subjects. 'Never inteded to say anything else :-)

Peter
by pbraub
Sun Dec 30, 2018 7:44 am
Forum: Photography Through the Microscope
Topic: Diatoms from Barbados
Replies: 13
Views: 2855

Anne,
thank you for these nice images. Your photography is - as always - very precise and to the point. I enjoy this very much.

Kind regards
Peter

I also enjoy that you include scalebars for your images!
by pbraub
Sat Dec 29, 2018 6:31 am
Forum: Photography Through the Microscope
Topic: Garden variety autofluorescence
Replies: 1
Views: 1285

Garden variety autofluorescence

Hi, i am toying around with my new UV excitation autofluorescence setup and took some garden variety images. Twig of a privet hedge (UPlanFL4x). http://www.photomacrography.net/forum/userpix/6661_lingu2_1.jpg Ghostberry (UPlanFL4x). http://www.photomacrography.net/forum/userpix/6661_ghostberry_1.jpg...
by pbraub
Fri Dec 21, 2018 1:40 am
Forum: Equipment Discussions
Topic: Unusual Olympus 10x
Replies: 9
Views: 3680

Seems like a reasonable explanation. Thank you Enrico and zzffnn!
by pbraub
Wed Dec 19, 2018 2:27 pm
Forum: Equipment Discussions
Topic: Unusual Olympus 10x
Replies: 9
Views: 3680

Do you by chance know why Olympus has a line of non "U" Apo objectives (e.g. the "PlanApo 60 1.40").
by pbraub
Tue Dec 18, 2018 11:44 am
Forum: Equipment Discussions
Topic: Unusual Olympus 10x
Replies: 9
Views: 3680

Unusual Olympus 10x

Hi, recently I came across an unusual 10x Olympus objective: A UPLanApo 10x Oil NA 0.40 and thought that it might be of interest to some members of the Forum. http://www.photomacrography.net/forum/userpix/6661_10xOil_1.jpg Oil immersion in a 10x is uncommon (to the point that I have never seen one) ...
by pbraub
Fri Dec 14, 2018 10:08 am
Forum: Community Members and Friends
Topic: Hi from northern Germany
Replies: 5
Views: 7502

George, Chris, thank you for your kind words. @ Chris DAPI and fading is a non-issue for me. I am not sure if you mean fading over time (storage) or photobleaching during illumination. The DAPI signal stays stable over months even at room temperature and i am sure that it will last even longer. Duri...
by pbraub
Thu Dec 13, 2018 12:47 pm
Forum: Equipment Discussions
Topic: Anatomy, A DIY Precision S & S System (First Light)
Replies: 27
Views: 7881

Hi, micro manager can directly interface with a wide variety of stages and other peripherals. See section Stages, filter wheels, shutters of the Micro Manager device support (1) for details. These are however mostly very expensive but from time to time and with patience you may get lucky on Ebay. I ...
by pbraub
Thu Dec 13, 2018 4:17 am
Forum: Community Members and Friends
Topic: Hi from northern Germany
Replies: 5
Views: 7502

Also some examples from recent work. This is more on the work than on the hobby side - but nevertheless taken on my setup: UPlanFl 60XOI @ NA 1.25 with HR-DIC on a dry NA 0.9 condenser taken with a Point Grey GS2 monochrome camera in Micro-Manager. The image is bronchial epithelium with a dual immun...