Search found 82 matches

by pbraub
Thu Sep 24, 2020 12:53 pm
Forum: Photography Through the Microscope
Topic: Diatoms DIC
Replies: 8
Views: 1754

Re: Diatoms DIC

Your result is very nice. Did you do any background subtraction or other correction?
by pbraub
Wed Sep 16, 2020 8:02 am
Forum: Equipment Discussions
Topic: "Disastrous" (completely unexpected!!) 100x oil immersion objective comparison
Replies: 38
Views: 5561

Re: "Disastrous" (completely unexpected!!) 100x oil immersion objective comparison

I share your impression that there is not much difference between the semi-apos and apos of olympus (especially the newer lines - (U)PLanFl N and UPlanSApo. The jump from achromats to flourites is obvious however (i use fl and apos on my diagnostic scope and would never go back). Some while ago i ha...
by pbraub
Thu Sep 10, 2020 11:51 pm
Forum: Equipment Discussions
Topic: Help needed: DIC components necessary for my Oly
Replies: 31
Views: 6862

Re: Help needed: DIC components necessary for my Oly

Thanks for the images! So that "blank slider" is merely one of the sliders without optics. I have a U-poc3 that looks similar. It probably has some retaining ring or similar to hold 25mm filters, but nothing fancy I would guess. A simple custom build part will probably do the trick. With 3D printin...
by pbraub
Wed Sep 09, 2020 12:26 pm
Forum: Equipment Discussions
Topic: Help needed: DIC components necessary for my Oly
Replies: 31
Views: 6862

Re: Help needed: DIC components necessary for my Oly

Got a similarly cooked one. It was left in the ligthpath of a 100W mercury burner. When you want to do epifluoresence and DIC you should use the u-an (instead of the u-ant) because it is outside of the illumination path. a) you don't lose excitation light and b) you don't cook your filter. Surprisin...
by pbraub
Sat Sep 05, 2020 4:37 am
Forum: Equipment Discussions
Topic: Help needed: DIC components necessary for my Oly
Replies: 31
Views: 6862

Re: Help needed: DIC components necessary for my Oly

The normal (non hc/hr) are the ones i use most often and are the ones I have in my condenser. They give an optimal middle ground imho. Ilike the HR prisms mostly for photography. When viewing by naked eye they seem very flat. In postprocessing you can get out so much more detail when adjusting the b...
by pbraub
Fri Sep 04, 2020 12:35 pm
Forum: Equipment Discussions
Topic: Help needed: DIC components necessary for my Oly
Replies: 31
Views: 6862

Re: Help needed: DIC components necessary for my Oly

"basic setup" restricted to the basic universal prism with the u-dict, cannot (officially) use dic in objectives marked with bfp1 (e.g. 60x and 100x apo). | | tube (e.g. u-tr30 or similar) | | |--| nosepiece with dic slider slot (d5re, d6re, d7re) and dic slider (U-DICT with U-ANT) objective sample ...
by pbraub
Fri Sep 04, 2020 12:17 pm
Forum: Equipment Discussions
Topic: Help needed: DIC components necessary for my Oly
Replies: 31
Views: 6862

Re: Help needed: DIC components necessary for my Oly

U-DICTS: S for shift. According to my digging, the difference for photomicrography isn't that huge. Using one requires an intermediate collar to hold a polariser, I guess the U-POT3? Complicates things, so not my type of joint here... bad puns are bad. (pot, yeah? hehehe I'll go stand in a corner) ...
by pbraub
Tue Mar 17, 2020 2:29 am
Forum: Equipment Discussions
Topic: Cleaning microscope lens surfaces
Replies: 9
Views: 2022

Hi, my experience with isoproanol is mixed, if you use it out of glass bottels it is fine, if you store it in plastic containers (falcon tubes) it leaves a white residue - i guess there is something dissolving in the isoprop our olympus service technician uses petroleum ether which evaporates nicely...
by pbraub
Thu Mar 05, 2020 4:40 am
Forum: Equipment Discussions
Topic: Advice for tethered shooting
Replies: 1
Views: 779

Advice for tethered shooting

Hi, we are setting up a new documentation station at work and I am currently looking into different options for doing photo documentation. Maximum image quality is not of concern (unfortunately) but ease of use. Ideally it should be some form of tethered shooting to PC with a rather robust camera. T...
by pbraub
Sat Feb 22, 2020 1:54 am
Forum: Equipment Discussions
Topic: Dry or wet darkfield condenser
Replies: 4
Views: 856

You have a full set of Phase objectives - Do you have the phase condenser (U-PCD)? it can be used with immersion medium and then - iirc - with condenser na of 1.25. It has an annulus for darkfield besides bf and ph1,2,3 (and with the 10x you can use the ph3 annulus as decent darkfield stop). With th...
by pbraub
Thu Aug 08, 2019 4:26 am
Forum: Equipment Discussions
Topic: Darkfield ring needed
Replies: 10
Views: 1893

@enricosavazzi
thank you for sharing this experience. i think that accuracy is key here. I agree, the price (incl s&h and import duties) is prettey steep for what you get.
by pbraub
Wed Aug 07, 2019 11:35 pm
Forum: Equipment Discussions
Topic: Darkfield ring needed
Replies: 10
Views: 1893

There is this guy on ebay selling a variety of condenser inserts in different sizes. I was contemplating of buying a set because i am also looking for an darkfield insert for the UCD8 but have never gotten around to.

https://www.ebay.com/itm/273087400339

Peter
by pbraub
Tue Jul 23, 2019 1:24 pm
Forum: Technical and Studio Photography -- Macro and Close-up
Topic: Mixite
Replies: 9
Views: 2404

Thank you for the beautiful image and the impressive stereo!
by pbraub
Mon Jul 15, 2019 11:35 pm
Forum: Equipment Discussions
Topic: Piezoelectric focuser on eBay
Replies: 76
Views: 13324

I also would be interested in the PCB (2 if possible). I would like to adapt one to micro-manager via their arduino device adapter.
by pbraub
Thu Jul 11, 2019 12:55 pm
Forum: Equipment Discussions
Topic: Piezoelectric focuser on eBay
Replies: 76
Views: 13324

Hi, i have also ordered one of these and would be very interested in participating!