Guercini, Here are a bunch where electronic flash was used to stop motion. https://www.photomacrography.net/forum/viewtopic.php?t=11702 https://www.photomacrography.net/forum/viewtopic.php?t=35158 https://www.photomacrography.net/forum/viewtopic.php?t=32234 https://www.photomacrography.net/forum/vie...

To check for coverage it will be fine. But the "B" coating reflects too high a percentage of light wavelengths up to about 500nm (see chart and spreadsheet here: https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=12767 ) I think you will find it does not give good color for imaging or light t...

Saul,

They show the "A" coating as in stock. Why not get the "A"?

I once ordered a "B" coated Thorlab condenser lens by accident, and it was unsuitable for use in the visible wavelengths.

Ken,
You have a knack for finding these. Such an odd life cycle. How long will a subject like this remain in good "photographic condition" if not disturbed?

Walter, You cut them without fixation on a vibratome? I suppose the tissue was strong enough to be sectioned? They were fresh subjects and were embedded in agarose which helped a great deal. Seeds were a problem with subjects like the "grape" tomato, because they would often get pushed through the s...

Sort of a plain wing, but I like the subdued interference colors that show up in various places, as well as the shape at the inner top. I think this a "thin film interference" (like in a soap bubble) and not the more familiar structural color found in beetles/butterflies caused by micro structures. ...

I've been very happy with the 50D that you suggested years ago too. It's still a very good microscope camera if you don't need video. As I mentioned, but will emphasize again, on a microscope all those pixels (32,5Mp) are really only of use in a quite limited number of cases. Mainly direct projecti...

I'm now using a 90D (APS-C) and an EOS-R (full frame). I changed primarily to have a fully electronic shutter in both full frame and APS-C. For continuous light this is fantastic and I would not want to go back to any type of mechanical shutter. But... the reason I asked about flash is that Olympus ...

What lighting do you typically use? Flash, continuous tungsten, continuous LED?

Let me know and I'll have a suggestion or two.

Really good looking 100X phase contrast! Interesting subject.

Good look. You found a nice clean one. So often they are covered in all sorts of debris.

Nice, put a print on your wall.

Fantastic!. The clarity really surprises me for circular oblique. Do I understand correctly... you used a 0.8 NA dry darkfield condenser? (In addition to the lens iris and condenser height).

These all came out great! First one particularly neat composition.

Looks quite good, rendered the fines hairs superbly. This is a great test subject. (What stacking method?) . Working distance is 0.7mm, so yes, this will be something to deal with! (I picked up a BD version awhile ago, but have barely played with yet).