Hi, Pau is right. The ELWD condensers are used on inverted microscopes. In the lab I work in we use a NA 0.3 elwd condenser with phase contrast on a small scope for checking cell cultures (Olympus CKX41) (this is commonly right next to the incubator in the lab for visual inspection - vitalitiy, cont...

You can also use (clear) nail varnish. However, it does not look as fancy as ringing cement.

Peter

This is an interesting piece of equipent! Does this also fit the Swing-Out type condenser (U-SC?)

Kind regards
Peter

Thank you for these nice pictures. I like the last one best - it is so calm and reduced to the essentials. It nearly looks like it could be tiled to infinity.

Peter

Anne,

I really like the crisp and clean look of your images. Fantastic. Thank you.

However no scalebar this time - i suppose for the sake of art.

Peter

Mazek,

thank you for the - again - wonderful photos and for the information on your setup!

Cheers
Peter

Maszek, thank you for these impressive images - I can even get the stereo images to fuse in my head (which I have some troubles with usually).

Is the FL pure UV excitation or do you use multiple wavelengths?

Cheers
Peter

I know. Thorlabs even advertises their composite breadboards with a technical note stating that more weight is not necessarily better.

However, the thought of doing macro on a running mill makes me smile. I can't help it.

ray_parkhurst wrote:Are your vibrations gone?

With the mill on or off that is?

Bill is rigth, this is definitely gall and not urinary bladder.

In Bills picture the mucosa is expanded by foamy cells (macrophages) under the epithelum. This is called "cholestatosis". Olympusmans pictures show gallbladder without any significant pathological changes.

Peter

The small dark cells are lymphocytes of the orginial lymphnote. They form swirling pattern around a germinal center. The cancer cells are the ones with a lighter red-ish color. Note how much more irregular the nuclei are. The lymphocyte nuclei are pretty much uniform where the neoplastic cells have ...

Do you have the option to use an higher NA objective to establish a ground truth?

Similar to this approach in microscopy (see figs 1 and 2)?

Peter

Thank you for these nice pictures. I am not sure, but the first three pictures seem to show the outer (molecular) layer of the cerebellum which has many argyrophile fibers and fewer cells. The last 3 pictures show Purkinje Cells sitting at the border of the nuclear (inner) layer and the outer molecu...

Well, I'd guess a longpass with appropriate cut-on frequency (it is the widest spectrum you can get in terms of emission). However they write on their hompage "... We employ a technique that we call “wide-spectrum fluorescence”..." and I am not 100% sure that they speak only about the barrier / emis...

@Pau
I was indeed referring to epifluorescence. Wouldnt you use a similar setup for "macro": Excitation filter on the UV Flashlight (for example) to cut any wavelengths higher than necessary and a longpass barrier filter. At least this would have been my approach.